PGM1 / YKL127W Overview
- Standard Name
- PGM1 1
- Systematic Name
- YKL127W
- SGD ID
- SGD:S000001610
- Feature Type
- ORF , Verified
- Description
- Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; PGM1 has a paralog, PGM2, that arose from the whole genome duplication 1 3
- Name Description
- PhosphoGlucoMutase 2
- Paralog
- PGM2 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Pgm1p is 570 amino acids long, low-to-moderate in abundance, of average half-life; contains 3 alpha-D-phosphohexomutase alpha/beta/alpha domains; acetylated on K507, phosphorylated on 6 residues
- Length (a.a.)
- 570
- Mol. Weight (Da)
- 63111.4
- Isoelectric Point
- 7.27
- Median Abundance (molecules/cell)
- 7371 +/- 3355
- Half-life (hr)
- 9.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PGM1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Cytoplasmic phosphoglucomutase involved in glucose metabolism, galactose catabolism, and glycogen and trehalose biosynthesis
View computational annotations
Molecular Function
- Manually Curated
- enables phosphoglucomutase activity (IGI)
Biological Process
- Manually Curated
- involved in galactose catabolic process (IGI)
- involved in glucose 1-phosphate metabolic process (IGI)
- involved in glucose 6-phosphate metabolic process (IGI)
- involved in glycogen biosynthetic process (IGI)
- involved in trehalose biosynthetic process (IGI)
- involved in UDP-alpha-D-glucose metabolic process (IGI)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants grow slowly, are sensitive to Calcofluor White, have some resistance to K2 killer toxin, grow well in glycerol or ethanol but not on synthetic complete; heterozygous diploid null displays chromosome instability
Classical Genetics
- null
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast PGM1 is homologous to human PGM1 and has been used to study mutations found in patients with PGM1-linked congenital disorder of glycosylation (PGM1-CDG), which is an autosomal recessive disease characterized by several phenotypes, some of which are life-threatening
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Pgm1p interacts physically with proteins involved in mitotic cell cycle, organelle fission, and DNA repair; PGM1 interacts genetically with genes involved in mitotic cell cycle and cytoskeleton organization; the pgm1 null mutant is viable, the null mutant of paralog pgm2 is viable, the pgm1 pgm2 double mutant is inviable or displays a growth defect
248 total interactions for 188 unique genes
Physical Interactions
- Affinity Capture-MS: 16
- Affinity Capture-RNA: 6
- Biochemical Activity: 4
- Co-localization: 1
- Co-purification: 1
- Proximity Label-MS: 2
- Reconstituted Complex: 1
Genetic Interactions
- Dosage Rescue: 7
- Negative Genetic: 189
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 1
- Positive Genetic: 12
- Synthetic Growth Defect: 1
- Synthetic Lethality: 3
- Synthetic Rescue: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 15
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2005-09-08
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.