PGM2 / YMR105C Overview


Standard Name
PGM2 1
Systematic Name
YMR105C
SGD ID
SGD:S000004711
Aliases
GAL5 17
Feature Type
ORF , Verified
Description
Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress; PGM2 has a paralog, PGM1, that arose from the whole genome duplication 1 2 4 5
Name Description
PhosphoGlucoMutase 3
Paralog
PGM1 5
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
PGM2/YMR105C is located on the right arm of chromosome XIII between replication origin ARS1317 and YPK2 protein kinase; coding sequence is 1713 nucleotides long with 16 SNPs, 3 of which cause amino acid polymorphisms; PGM2 has a paralog, PGM1, that arose from the whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Pgm2p is 569 amino acids long, moderate in abundance, very long-lived (3x average); contains 3 alpha-D-phosphohexomutase alpha/beta/alpha domains; succinylated on K544, acetylated on K428, K449, and K537, phosphorylated on 8 residues; protein abundance increases in response to DNA replication stress
Length (a.a.)
569
Mol. Weight (Da)
63084.0
Isoelectric Point
6.59
Median Abundance (molecules/cell)
21225 +/- 17383
Half-life (hr)
30.5

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all PGM2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Cytoplasmic phosphoglucomutase involved in metabolism of glycogen, trehalose, UDP-glucose, and glucose 1-phosphate; also involved in calcium homeostasis

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated

Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null grows slowly, is heat sensitive, has increased replicative lifespan but decreased chronological lifespan, cannot grow on galactose, shows increased calcium uptake, decreased resistance to Calcofluor White, cyclosporin, SDS, and caffeine
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast PGM2 is homologous to human PGM1 and has been used to study mutations found in patients with PGM1-linked congenital disorder of glycosylation (PGM1-CDG), which is an autosomal recessive disease characterized by several phenotypes, some of which are life-threatening
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Pgm2p interacts physically with proteins involved in mitotic cell cycle and DNA repair; PGM2 interacts genetically with genes involved in lipid metabolism and carbohydrate metabolism; the pgm2 null mutant is viable, the null mutant of paralog pgm1 is viable, the pgm2 pgm1 double mutant is inviable or displays a growth defect

159 total interactions for 127 unique genes

Physical Interactions

  • Affinity Capture-MS: 26
  • Affinity Capture-RNA: 3
  • PCA: 13
  • Reconstituted Complex: 1

Genetic Interactions

  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 87
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 1
  • Positive Genetic: 15
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 2
  • Synthetic Rescue: 2
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
PGM2 transcription is upregulated by Hsf1p in response to heat; PGM2 translation is upregulated by Yta6p and Ypr096cp in response to lithium; Pgm2 protein activity is regulated by Rad53p
Regulators
19
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2005-09-08

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
46
Additional
125
Reviews
17

Resources