YAP5 / YIR018W Overview

Standard Name
YAP5 1
Systematic Name
Feature Type
ORF , Verified
Basic leucine zipper (bZIP) iron-sensing transcription factor; senses high-iron conditions via two Fe/S clusters bound to its activator domain; involved in diauxic shift; YAP5 has a paralog, YAP7, that arose from the whole genome duplication 1 2 3 4 5
Name Description
Yeast AP-1 1
YAP7 3
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

YAP5 has a paralog, YAP7, that arose from the whole genome duplication
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
472 +/- 234


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all YAP5 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Sequence-specific DNA binding transcription factor involved in the regulation of transcription by RNA polymerase II in response to iron; also involved in the regulation of telomeric chromatin silencing

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutants exhibit increased fitness and resistance to antimalarial quinine, abnormal vacuolar morphology, impaired filamentous growth and use of various nitrogen sources; homozygous diploid nulls are sensitive to preservative sorbate and heat shock; heterozygous diploid nulls are haploinsufficient; overexpression increases filamentous growth
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

The yap5 null mutant is viable; the null mutant of paralog yap7 is viable; the yap5 yap7 double mutant has not been annotated for phenotype.

56 total interactions for 46 unique genes

Physical Interactions

  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 8
  • FRET: 1
  • PCA: 1
  • Protein-peptide: 1
  • Proximity Label-MS: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 6

Genetic Interactions

  • Dosage Rescue: 3
  • Negative Genetic: 16
  • Phenotypic Enhancement: 7
  • Positive Genetic: 5
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

YAP5 encodes an AP-1 type transcription factor which contains a basic leucine zipper domain (bZIP), and binds to a consensus cis-acting Yap recognition element (YRE; 5'-TTAC/GTAA-3'). Yap5p is the only member of the Yap family that regulates the expression of vacuolar iron transport in response to cytosolic iron, and is responsible for the high-iron induction of CCC1, which encodes a vacuolar iron transporter. Yap5p is constitutively localized to the nucleus even in the absense of iron, and binding of Yap5p to the CCC1 promoter is iron independent, but transcriptional activation does require iron. Yap5p regulates the expression of glutaredoxin GRX4 in a similar manner; binding to its promoter is iron-independent, while activation of transcription is iron-dependent. Yap5p also mediates induction of TYW1, a gene that encodes an iron-sulfur cluster enzyme that participates in the synthesis of wybutosine modified tRNA, thereby providing cellular protection from high iron toxicity by the consumption of free cytosolic iron through the formation of protein-bound iron-sulfur clusters. A limited number of other genes have also been identified as Yap5p targets: CUP1, YHB1, PHO3, and GDH1.
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.