ADH2 / YMR303C Overview


Standard Name
ADH2 1
Systematic Name
YMR303C
SGD ID
SGD:S000004918
Aliases
ADR2 5
Feature Type
ORF , Verified
Description
Glucose-repressible alcohol dehydrogenase II; catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 2 3 4
Name Description
Alcohol DeHydrogenase
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
ADH2 is located on the right arm of chromosome XIII near the telomere between YME2 mitochondrial membrane protein and UBP15 ubiquitin-specific protease; coding sequence is 1047 nucleotides long with 59 SNPs, 22 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Adh2p is 348 amino acids long, shorter-lived, low in abundance; ubiquitinylated on 4 lysines, sumoylated on 10 lysines, succinylated on K28 and K315, phosphorylated on 9 residues
Length (a.a.)
348
Mol. Weight (Da)
36728.3
Isoelectric Point
6.73
Median Abundance (molecules/cell)
2376 +/- 1478
Half-life (hr)
8.5

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all ADH2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Cytoplasmic NAD-dependent alcohol dehydrogenase that converts ethanol to acetylaldehyde

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant accumulates hydrogen peroxide and superoxide and is resistant to allyl alcohol; overexpression stimulates filamentous growth
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Adh2p interacts physically with proteins involved in mitochondrion organization and mitotic cell cycle; ADH2 interacts genetically with genes involved in amino acid metabolism

95 total interactions for 89 unique genes

Physical Interactions

  • Affinity Capture-MS: 28
  • Affinity Capture-RNA: 1
  • Co-fractionation: 1
  • Co-localization: 1
  • Protein-peptide: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions

  • Negative Genetic: 34
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 1
  • Positive Genetic: 16
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
ADH2 promoter is bound by Fkh1p and Sua7p in response to heat; ADH2 transcription is downregulated by Ixr1p in response to hypoxia; ADH2 transcription is downregulated by Snf5p in presence of glucose and upregulated by Snf5p in response to glucose starvation
Regulators
16
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2008-08-19

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
102
Additional
346
Reviews
60

Resources