SNF5 / YBR289W Overview

Standard Name
SNF5 1
Systematic Name
HAF4 60 , SWI10 14 , TYE4 61 62
Feature Type
ORF , Verified
Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions 2 3 5
Name Description
Sucrose NonFermenting 4
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

SNF5 is located on the right arm of chromosome II near the telomere between APM3 Mu3-like subunit of the clathrin associated protein complex and BSD2 heavy metal ion homeostasis protein; coding sequence is 2718 nucleotides long with 38 SNPs and several microsatellites
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Snf5p is 905 amino acids long, low in abundance; contains multiple polyglutamine repeats (QQQ...), 151 of the 905 residues are glutamine; acetylated on K350, ubiquitinylated on K154 and K447, phosphorylated on 9 residues
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
1956 +/- 832


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all SNF5 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

DNA-binding and transcription-factor-binding subunit of the SWI/SNF chromatin remodelling complex, which disrupts chromatin structure to enhance binding of transcription factors; involved in carbon catabolite activation of RNA Pol II transcription, double-strand break repair, and activation of invasive growth in response to glucose limitation; localizes to the nucleus and to the cytosol during response to hypoxia

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant displays slow growth, UV sensitivity, defects in using glutamate as nitrogen source, increased cell size, small nucleolus, overexpanded ER with disorganized cytosolic structures, decreased competitive growth, decreased chronological lifespan, abnormal sporulation, decreased respiratory growth, and sensitivity to hydroxyurea, methyl methanesulfonate, azoles, various antifungals, various metals, heat, Congo Red, sorbate, boric acid, caffeine, cycloheximide
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Snf5p interacts physically with proteins involved in transcription; SNF5 interacts genetically with genes involved in transcription

688 total interactions for 518 unique genes

Physical Interactions

  • Affinity Capture-MS: 106
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 32
  • Co-crystal Structure: 2
  • Co-localization: 2
  • Co-purification: 5
  • PCA: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 3

Genetic Interactions

  • Dosage Growth Defect: 2
  • Dosage Lethality: 2
  • Dosage Rescue: 1
  • Negative Genetic: 290
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 6
  • Positive Genetic: 186
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 11
  • Synthetic Rescue: 2
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

SNF5 promoter is bound by Fkh1p; SNF5 promoter is bound by Xbp1p in response to heat; SNF5 transcription is regulated by Spt10p; SNF5 transcription is downregulated by Ixr1p in response to hypoxia
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2006-03-27

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.