FKH1 / YIL131C Overview
- Standard Name
- Systematic Name
- SGD ID
- Feature Type
Forkhead family transcription factor; rate-limiting replication origin activator; evolutionarily conserved lifespan regulator; binds multiple chromosomal elements with distinct specificities, cell cycle dynamics; regulates transcription elongation, chromatin silencing at mating loci, expression of G2/M phase genes; facilitates clustering, activation of early-firing replication origins; binds HML recombination enhancer, regulates donor preference during mating-type switching
- Name Description
- ForK head Homolog
- Comparative Info
The S. cerevisiae Reference Genome sequence is derived from laboratory strain
S288C. Download DNA or protein sequence, view genomic context and
coordinates. Click "Sequence Details" to view all sequence information for this locus, including that
for other strains.
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- Mol. Weight (Da)
- Isoelectric Point
- Median Abundance (molecules/cell)
- 1771 +/- 891
- Half-life (hr)
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
Gene Ontology Details
GO Annotations consist of four mandatory components: a gene product, a term from one of the three
Gene Ontology (GO) controlled vocabularies
Biological Process, and
Cellular Component), a reference, and an
evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the
literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view
all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Sequence-specific DNA binding transcription factor involved in chromatin remodeling, mitotic transcription regulation, transcription termination, mating-type switching, and pseudohyphal growth; binds DNA replication origins and positively regulates replication initiation; also binds centromeres
View computational annotations
- Manually Curated
- Manually Curated
- Manually Curated
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable
(e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background,
and a reference. In addition, annotations are classified as classical genetics or high-throughput
(e.g., large scale survey, systematic mutation set). Whenever possible, allele information and
additional details are provided. Click "Phenotype Details" to view all phenotype annotations and
evidence for this locus as well as phenotypes it shares with other genes.
- Non-essential gene; null mutants have abnormal vacuolar morphology, reduced silencing at mating-type loci and abnormal mating-type switching; nulls are also sensitive to heat, and exhibit altered rates of cell cycle progression through the S and G2/M phases; overexpression slows growth
Interaction annotations are curated by BioGRID and include physical
or genetic interactions observed
between at least two genes. An interaction annotation is composed of the interaction type, name of the
interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a
reference, as well as other experimental details. Click "Interaction Details" to view all interaction
annotations and evidence for this locus, including an interaction visualization.
- The fkh1 null mutant is viable; the null mutant of paralog fkh2 is viable; the fkh1 fkh2 double mutant is inviable.
200 total interactions for 136 unique genes
- Affinity Capture-MS: 34
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 20
- Co-localization: 1
- Co-purification: 1
- PCA: 2
- Reconstituted Complex: 5
- Two-hybrid: 9
- Dosage Growth Defect: 4
- Dosage Rescue: 4
- Negative Genetic: 51
- Phenotypic Enhancement: 21
- Phenotypic Suppression: 11
- Positive Genetic: 25
- Synthetic Growth Defect: 5
- Synthetic Lethality: 1
- Synthetic Rescue: 2
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the
given locus, based on experimental evidence. This evidence includes data generated through
high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO
enrichment among regulation Targets, and a regulator/target diagram for the locus.
- FKH1 encodes a transcription factor that is a member of the widely conserved eukaryotic forkhead family of proteins, which contain the characteristic forkhead (FH), or winged helix, DNA-binding domain. Many forkhead proteins also have a second domain referred to as the forkhead-associated (FHA) domain which binds phosphorylated threonine residues within proteins. Fkh1p has both FH and FHA domains, and is involved in regulation of expression of the CLB2 cluster of genes during the G2/M phase of the mitotic cell cycle. The CLB2 cluster of genes includes mitotic regulators such as CLB1, CLB2, CDC5 and CDC20, as well as SWI5 and ACE2, transcription factors required for the subsequent temporal wave of cell cycle regulated gene expression in the M/G1 phase interval. Fkh1p cooperates with Isw1p to remodel chromatin and repress transcription of CLB2 during G2/M. Fkh1p associates with coding regions of active genes to regulate transcription by affecting the phosphorylation status of the C-terminal repeat domain (CTD) of RNA Polymerase II. The Fkh1p binding site 5'-GTAAACAAA-3' is present in over 1400 locations throughout the genome. FKH1 has additional roles in the establishment of chromatin silencing at the silent mating-type cassette and in the regulation of donor preference during mating type switching. FKH1 and the Swi4p/Swi6p-containing SCB-binding factor (SBF) independently regulate donor preference through direct interactions with a cis-acting sequence called the recombination enhancer, with Fkh1p binding in the G2 phase and SBF binding in the G1 phase of the cell cycle.
Expression data are derived from records contained in the
Gene Expression Omnibus (GEO), and are first log2
transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result
there may be a greater number of conditions than datasets represented in a single clickable histogram
bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from
those that are up-regulated (red). Click "Expression Details" to view all expression annotations and
details for this locus, including a visualization of genes that share a similar expression pattern.
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links
to gene names and curated GO terms are included within the Summary Paragraphs.
All manually curated literature for the specified gene, organized into topics according to their
relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details"
to view all literature information for this locus, including shared literature between genes.