CDC20 / YGL116W Overview


Standard Name
CDC20 1
Systematic Name
YGL116W
SGD ID
SGD:S000003084
Aliases
PAC5 15
Feature Type
ORF , Verified
Description
Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress 2
Name Description
Cell Division Cycle 3
Comparative Info
Integrated model organism details available at the Alliance of Genome Resources website
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
610
Mol. Weight (Da)
67371.8
Isoelectric Point
9.11
Median Abundance (molecules/cell)
2175 +/- 1196
Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Ubiquitin-protein transferase activator; binds and activates the anaphase-promoting complex (APC); involved in APC-dependent catabolism, regulation of mitotic metaphase/anaphase transition, and regulation of meiotic nuclear division; involved in the mitotic spindle assembly checkpoint and is also a subunit of the mitotic checkpoint complex

View computational annotations

Cellular Component

Manually Curated
High-Throughput

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene; conditional mutations cause cell cycle arrest in M phase and accumulation of abnormally large budded cells
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast CDC20 is homologous to human CDC20, and has been used to study melanoma

Manually Curated

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


1582 total interactions for 1002 unique genes

Physical Interactions

  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 35
  • Biochemical Activity: 20
  • Co-fractionation: 4
  • Co-localization: 3
  • Co-purification: 2
  • Reconstituted Complex: 9
  • Two-hybrid: 18

Genetic Interactions

  • Dosage Lethality: 2
  • Dosage Rescue: 9
  • Negative Genetic: 928
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 15
  • Positive Genetic: 200
  • Synthetic Growth Defect: 191
  • Synthetic Lethality: 102
  • Synthetic Rescue: 11
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
8
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2005-05-11

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
114
Additional
188
Reviews
87

Resources