STP1 / YDR463W Overview
- Standard Name
- Systematic Name
- SGD ID
- Feature Type
Transcription factor; contains a N-terminal regulatory motif (RI) that acts as a cytoplasmic retention determinant and as an Asi dependent degron in the nucleus; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication
- Name Description
- Species-specific tRNA Processing
- Comparative Info
The S. cerevisiae Reference Genome sequence is derived from laboratory strain
S288C. Download DNA or protein sequence, view genomic context and
coordinates. Click "Sequence Details" to view all sequence information for this locus, including that
for other strains.
- STP1 has a paralog, STP2, that arose from the whole genome duplication
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- Mol. Weight (Da)
- Isoelectric Point
- Median Abundance (molecules/cell)
- 1005 +/- 437
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
Gene Ontology Details
GO Annotations consist of four mandatory components: a gene product, a term from one of the three
Gene Ontology (GO) controlled vocabularies
Biological Process, and
Cellular Component), a reference, and an
evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the
literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view
all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Sequence-specific DNA binding RNA polymerase II transcription activator; localizes to nucleus, cytoplasm, and plasma membrane
View computational annotations
- Manually Curated
- Manually Curated
- Manually Curated
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable
(e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background,
and a reference. In addition, annotations are classified as classical genetics or high-throughput
(e.g., large scale survey, systematic mutation set). Whenever possible, allele information and
additional details are provided. Click "Phenotype Details" to view all phenotype annotations and
evidence for this locus as well as phenotypes it shares with other genes.
Interaction annotations are curated by BioGRID and include physical
or genetic interactions observed
between at least two genes. An interaction annotation is composed of the interaction type, name of the
interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a
reference, as well as other experimental details. Click "Interaction Details" to view all interaction
annotations and evidence for this locus, including an interaction visualization.
- The stp1 null mutant is viable; the null mutant of paralog stp2 is viable; the stp1 stp2 double mutant displays growth defects and aberrations in gene expression; the stp1 stp2 gap1 triple mutant is inviable.
256 total interactions for 214 unique genes
- Affinity Capture-MS: 7
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 2
- Biochemical Activity: 1
- Co-purification: 1
- Two-hybrid: 2
- Dosage Rescue: 2
- Negative Genetic: 188
- Phenotypic Enhancement: 6
- Phenotypic Suppression: 1
- Positive Genetic: 24
- Synthetic Growth Defect: 14
- Synthetic Lethality: 3
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the
given locus, based on experimental evidence. This evidence includes data generated through
high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO
enrichment among regulation Targets, and a regulator/target diagram for the locus.
- STP2 and its paralog STP1 encode transcription factors that are members of the C2H2 zinc finger class. Stp1p and Stp2p act independently of each other but have redundant roles in regulation of genes encoding amino acid and peptide permeases, including AGP1, BAP2, BAP3, DIP5, GNP1, MUP1, TAT1 and TAT2, and PTR2. In response to the presence of extracellular amino acids, they bind to UAS-aa elements in the promoters of their target genes and activate transcription. Stp1p and Stp2p are among several transcription factors involved in nitrogen source utilization (including Uga3p, Dal82p, Stp1p, and Stp2p) whose function depends on cooperation with the transcription factor Dal81p. In the case of Stp1p and Stp2p, Dal81p facilitates their promoter binding and contributes to transcription activation, but is not absolutely required for activity. The involvement of Dal81p in pathways for utilization of several different nitrogen sources allows the cell to integrate the disparate nutritional signals and to induce different pathways according to availability of each source. Activity of Stp1p and Stp2p is regulated by the SPS-sensing pathway, so-called because its first step is sensing by a plasma membrane complex composed of Ssy1p, Ptr3, and Ssy5p. In the absence of extracellular amino acids, Stp1p and Stp2p exist as precursor proteins that are largely cytoplasmic. The small amounts of precursor that do enter the nucleus are prevented from binding to target promoters by three nuclear inner membrane proteins, Asi1p, Asi2p, and Asi3p. Under inducing conditions, the proteolytic activity of Ssy5p is activated and it cleaves inhibitory N-terminal domains from Stp1p and Stp2p. The mature forms of Stp1p and Stp2p are able to enter the nucleus and activate transcription.
Expression data are derived from records contained in the
Gene Expression Omnibus (GEO), and are first log2
transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result
there may be a greater number of conditions than datasets represented in a single clickable histogram
bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from
those that are up-regulated (red). Click "Expression Details" to view all expression annotations and
details for this locus, including a visualization of genes that share a similar expression pattern.
All manually curated literature for the specified gene, organized into topics according to their
relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details"
to view all literature information for this locus, including shared literature between genes.