DAL81 / YIR023W Overview


Standard Name
DAL81
Systematic Name
YIR023W
SGD ID
SGD:S000001462
Aliases
UGA35 3 , DURL 3
Feature Type
ORF , Verified
Description
Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism 1 2
Name Description
Degradation of Allantoin
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
970
Mol. Weight (Da)
109179.7
Isoelectric Point
8.11
Median Abundance (molecules/cell)
1533 +/- 944

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all DAL81 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
A RNA polymerase II transcription coactivator involved in activation of transcription from RNA polymerase II promoter, positive regulation of urea catabolic process, and RNA polymerase II transcriptional preinitiation complex assembly; localized to nucleus

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutation causes decreased expression of multiple genes controlled by nitrogen catabolite repression system; reduction of function results in decreased ability to utilize urea and gamma-aminobutyrate as nitrogen sources; in systematic studies mutants are sensitive to both acidic and alkaline conditions, also sensitive to numerous chemicals and accumulate free amino acids
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


680 total interactions for 583 unique genes

Physical Interactions

  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 6
  • Biochemical Activity: 1
  • Co-localization: 1
  • PCA: 1
  • Two-hybrid: 4

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Negative Genetic: 510
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 120
  • Synthetic Growth Defect: 23
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
DAL81 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Dal81p is required for transcriptional activation of many genes involved in nitrogen source utilization, always in cooperation with another transcription factor specific for a particular nitrogen source. It has regulates utilization of gamma-aminobutyrate (GABA), together with Uga3p; allantoin, with Dal82p; and amino acids, with Stp1p and Stp2p. This common requirement for Dal81p in the pathways for utilization of several different nitrogen sources allows the cell to integrate the disparate nutritional signals and to induce different pathways according to availability of each source. Although Dal81p has a putative DNA-binding domain, it is unclear whether it binds DNA in vivo, since this domain is not required for transcriptional activation with at least some of its partners (Uga3p and Dal82p). Furthermore, Dal81p has been shown to interact physically with Dal82p, so its mechanism of action may involve binding to its partner transcription factor rather than to DNA.
Regulators
5
Targets
22
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
39
Additional
57
Reviews
10

Resources