TFC3 / YAL001C Overview

Standard Name
TFC3 1
Systematic Name
FUN24 6 , TSV115 7 8 , tau 138 1
Feature Type
ORF , Verified
Subunit of RNA polymerase III transcription initiation factor complex; part of TauB domain of TFIIIC that binds DNA at BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding; largest of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); colocalizes with condensin at pol III genes and several ETC (extra TFIIIC) sites; may have a role in recruiting or stabilizing Scc2/4 and condensin on chromosomes 1 2 3 4 5
Name Description
Transcription Factor class C 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

TFC3 is located on the left arm of chromosome I between VPS8 CORVET complex subunit and NUP60 FG-nucleoporin; coding sequence is 3573 nucleotides long with 1 intronic SNP, 6 nonsynonymous SNPs, 12 synonymous SNPs
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Subunit of RNA polymerase III transcription initiation factor complex; part of TauB domain of TFIIIC that binds DNA
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
1809 +/- 1003
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all TFC3 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Subunit of the transcription factor TFIIIC complex; involved in recruiting TFIIIB to chromatin for transcription of RNA genes by RNA polymerase III

View computational annotations

Biological Process

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Essential gene; conditional mutants display abnormal protein distribution of condensin complex subunit proteins, Brn1p and Scc4p resulting in decreased association of these proteins with chromosomes; heterozygous null mutant shows a decreased rate of growth
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

239 total interactions for 179 unique genes

Physical Interactions

  • Affinity Capture-MS: 34
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 14
  • Co-fractionation: 1
  • Co-localization: 1
  • Co-purification: 1
  • Far Western: 1

Genetic Interactions

  • Dosage Lethality: 1
  • Dosage Rescue: 16
  • Negative Genetic: 140
  • Positive Genetic: 21
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

TFC3 encodes one of six subunits of RNA polymerase III (pol III) transcription factor TFIIIC, which is composed of Tfc1p, Tfc3p, Tfc4p, Tfc6p, Tfc7p, and Tfc8p. Genes that are transcribed by pol III, all encoding small RNAs such as tRNAs, 5S rRNA genes, and U6 spliceosomal RNA, contain two conserved promoter elements termed the A and B boxes. The A box is bound by the TA subcomplex of TFIIIC, and the B box is bound by the TB subcomplex. Tfc3p is part of the TB subcomplex of TFIIIC, along with Tfc6p and Tfc8p, and is important for recognition of and binding to the B box. During pol III transcription initiation, TFIIIC first binds to DNA and then recruits the subunits of TFIIIB: Brf1p, Bdp1p, and Spt15p (also known as TATA-binding protein). The major role of TFIIIC is to facilitate the assembly of TFIIIB, which is a requirement for pol III transcription initiation. TFIIIC is also involved in transcription reinitiation by pol III, and its binding to chromatin may have a regulatory role in the determination of chromatin structure.
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.