Reference: Memisoglu G, et al. (2019) PP2C phosphatases promote autophagy by dephosphorylation of the Atg1 complex. Proc Natl Acad Sci U S A 116(5):1613-1620

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Abstract


Macroautophagy is orchestrated by the ATG1-ATG13 complex in budding yeast. Under nutrient-rich conditions, ATG13 is maintained in a hyperphosphorylated state by the TORC1 kinase. After nutrient starvation, ATG13 is dephosphorylated, triggering ATG1 kinase activity and macroautophagy induction. The phosphatases that dephosphorylate ATG13 remain uncharacterized. Here, we show that two redundant PP2C phosphatases, PTC2 and PTC3, regulate macroautophagy by dephosphorylating ATG13 and ATG1. In the absence of these phosphatases, starvation-induced macroautophagy and the cytoplasm-to-vacuole targeting pathway are inhibited, and the recruitment of the essential autophagy machinery to the phagophore assembly site is impaired. Expressing a genomic ATG13 -8SA allele lacking key TORC1 phosphorylation sites partially bypasses the macroautophagy defect in ptc2Delta ptc3Delta strains. Moreover, PTC2 and PTC3 interact with the ATG1-ATG13 complex. Taken together, these results suggest that PP2C-type phosphatases promote macroautophagy by regulating the ATG1 complex.

Reference Type
Journal Article | Research Support, N.I.H., Extramural
Authors
Memisoglu G, Eapen VV, Yang Y, Klionsky DJ, Haber JE
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