Reference: Kimpe M, et al. (2012)
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Abstract
The yeast Saccharomyces cerevisiae responds to amino acid deprivation by increasing translation of the transcription factor Gcn4, which enhances expression of amino acid biosynthetic genes. Accumulation of uncharged tRNAs activates the Gcn2 protein kinase, which phosphorylates the alpha subunit of the eukaryotic initiation factor 2 (eIF2α). The resulting downregulation of eIF2 activity causes reduction of general translation and stimulation of GCN4 translation. S. cerevisiae contains three PDK1 orthologs (encoded by PKH1, PKH2 and PKH3) that have been implicated in nutrient signaling. Using heterologously expressed proteins, we demonstrate physical interaction between Pkh1 and all three subunits of eIF2 as well as Gcn2. We confirm the interaction between Pkh1 and Gcn2 by co-immunoprecipitation in yeast cell extracts and show that Pkh1 can phosphorylate Gcn2 in vitro. However, Pkh1 inactivation did not affect eIF2α-S51 phosphorylation in vivo or GCN4 translation in response to amino acid deprivation. Hence, the physiological importance of the close interactions between Pkh1 and Gcn2 or eIF2 could depend on other conditions and/or other targets of the Gcn2/eIF2 system.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't
- Authors
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Kimpe M,
Voordeckers K,
Thevelein JM,
Van Zeebroeck G
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- SUI2 | GCN2 | PKH1 | Eukaryotic translation initiation factor 2 complex
- GCD11 | SCH9 | TPK1 | SUI3
Gene Ontology Annotations
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Evidence ID |
Analyze ID |
Gene/Complex |
Systematic Name/Complex Accession |
Qualifier |
Gene Ontology Term ID |
Gene Ontology Term |
Aspect |
Annotation Extension |
Evidence |
Method |
Source |
Assigned On |
Reference |
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Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Phenotype |
Experiment Type |
Experiment Type Category |
Mutant Information |
Strain Background |
Chemical |
Details |
Reference |
Disease Annotations
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Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Disease Ontology Term |
Disease Ontology Term ID |
Qualifier |
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Assigned On |
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Reference |
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Evidence ID |
Analyze ID |
Regulator |
Regulator Systematic Name |
Target |
Target Systematic Name |
Direction |
Regulation of |
Happens During |
Regulator Type |
Direction |
Regulation Of |
Happens During |
Method |
Evidence |
Strain Background |
Reference |
Post-translational Modifications
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Genetic Interactions
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Evidence ID |
Analyze ID |
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Interactor |
Interactor Systematic Name |
Interactor |
Interactor Systematic Name |
Allele |
Assay |
Annotation |
Action |
Phenotype |
SGA score |
P-value |
Source |
Reference |
Note |
Physical Interactions
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genes involved in the interaction.
| Interactor | Interactor | Assay | Annotation | Action | Modification |
| GCD11 | PKH1 | Reconstituted Complex | manually curated | Bait-Hit | No Modification |
| GCD11 | SCH9 | Reconstituted Complex | manually curated | Bait-Hit | No Modification |
| GCD11 | TPK1 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
| GCD11 | PKH1 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
| GCD11 | SCH9 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
| GCN2 | PKH1 | Reconstituted Complex | manually curated | Bait-Hit | No Modification |
| GCN2 | PKH1 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
| GCN2 | PKH1 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| GCN2 | PKH1 | Biochemical Activity | manually curated | Hit-Bait | phosphorylated residue |
| PKH1 | SUI2 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
Functional Complementation Annotations
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Complement ID |
Locus ID |
Gene |
Species |
Gene ID |
Strain background |
Direction |
Details |
Source |
Reference |