PKH2 / YOL100W Overview


Standard Name
PKH2 1
Systematic Name
YOL100W
SGD ID
SGD:S000005460
Feature Type
ORF , Verified
Description
Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication 1 2 3 4 5 6
Name Description
Pkb-activating Kinase Homolog 1
Paralog
PKH1 6
Comparative Info
Integrated model organism details available at the Alliance of Genome Resources website
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
1081
Mol. Weight (Da)
121717.0
Isoelectric Point
10.23
Median Abundance (molecules/cell)
515 +/- 286
Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Serine/threonine protein kinase; component of the MAPK cascade that regulates the cell wall integrity pathway by activating Ypk2p; regulates late endosome to vacuole transport via the MVB sorting pathway through Vps27p phosphorylation; regulates assembly of the cytoplasmic mRNA processing body (P-body) and mRNA decay via poly(A) tail shortening; involved in Rps6a/b phosphorylation; localizes to the cell cortex, the cytosol and colocalizes with eicosomes

View computational annotations

Molecular Function

Manually Curated
High-Throughput

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant shows increased innate thermotolerance, chronological and replicative lifespan, and competitive fitness; homozygous null mutation in diploid decreases sporulation
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The pkh2 null mutant is inviable in the CEN.PK background, but viable in the S288C background; the null mutant of paralog pkh1 is viable; the pkh2 pkh1 double mutant is inviable or displays a growth defect.

111 total interactions for 91 unique genes

Physical Interactions

  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 1
  • Biochemical Activity: 8
  • PCA: 7
  • Reconstituted Complex: 37
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Rescue: 1
  • Negative Genetic: 17
  • Phenotypic Enhancement: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 5
  • Synthetic Rescue: 2
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
5
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
37
Additional
34
Reviews
39

Resources