Reference: Kim M, et al. (2009) Phosphorylation of the yeast Rpb1 C-terminal domain at serines 2, 5, and 7. J Biol Chem 284(39):26421-6

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Abstract


The C-terminal domain (CTD) of Rpb1, the largest subunit of RNA polymerase II, acts as a binding platform for various mRNA processing and histone-modifying enzymes that act co-transcriptionally. These factors are targeted to specific phosphorylation states of the CTD that predominate at different stages of transcription. Within the repeating sequence YSPTSPS, serines 2 and 5 are major phosphorylation sites, but serine 7 phosphorylation was recently discovered in mammalian cells. Here we show that CTD serine 7 is also phosphorylated in yeast and that Ser-7(P) chromatin immunoprecipitation patterns resemble those of Ser-5(P). The basal factor TFIIH can phosphorylate Ser-7 in vitro and is necessary for Ser-7(P) in vivo. Interestingly, deletion of the CTD Ser-5(P) phosphatase Rtr1 leads to an increase in Ser-5(P) but not Ser-7(P).

Reference Type
Journal Article | Research Support, N.I.H., Extramural
Authors
Kim M, Suh H, Cho EJ, Buratowski S
Primary Lit For
SSL2 | TFB1 | KIN28 | TFB5 | RPO21 | RAD3 | SSL1 | TFB4 | TFB3 | CCL1 | RTR1 | ... Show all
Additional Lit For
kin28-as

Gene Ontology Annotations 2 entries for 1 gene


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
General transcription factor TFIIH complexinvolved inphosphorylation of RNA polymerase II C-terminal domainIDAComplexPortal2017-04-12
General transcription factor TFIIH complexenablesRNA polymerase II CTD heptapeptide repeat kinase activityIDAComplexPortal2017-04-12
Showing 1 to 2 of 2 entries

Phenotype Annotations 1 entry for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
KIN28protein/peptide modification: decreased
Reporter: Rpo21p
classical genetics conditional
Allele: kin28-as

L83G; bulky residue in the ATP binding pocket mutated to glycine, resulting in an enlarged pocket; analog-sensitive allele

Other10 μM 1-NM-PP1Details: phosphorylation of serine 5 in the CTD is greatly reduced and serine 7 phosphorylation is reduced to near background levels
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Regulation Annotations 10 entries for 11 genes


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RegulatorTargetDirectionRegulation OfHappens DuringMethodEvidence
KIN28RPO21protein activitymanually curatedprotein kinase assay evidence
TFB5RPO21protein activitymanually curatedprotein kinase assay evidence
TFB1RPO21protein activitymanually curatedprotein kinase assay evidence
TFB3RPO21protein activitymanually curatedprotein kinase assay evidence
RAD3RPO21protein activitymanually curatedprotein kinase assay evidence
SSL2RPO21protein activitymanually curatedprotein kinase assay evidence
SSL1RPO21protein activitymanually curatedprotein kinase assay evidence
TFB2RPO21protein activitymanually curatedprotein kinase assay evidence
CCL1RPO21protein activitymanually curatedprotein kinase assay evidence
TFB4RPO21protein activitymanually curatedprotein kinase assay evidence
Showing 1 to 10 of 10 entries