The C-terminal domain (CTD) of Rpb1, the largest subunit of RNA polymerase II, acts as a binding platform for various mRNA processing and histone-modifying enzymes that act co-transcriptionally. These factors are targeted to specific phosphorylation states of the CTD that predominate at different stages of transcription. Within the repeating sequence YSPTSPS, serines 2 and 5 are major phosphorylation sites, but serine 7 phosphorylation was recently discovered in mammalian cells. Here we show that CTD serine 7 is also phosphorylated in yeast and that Ser-7(P) chromatin immunoprecipitation patterns resemble those of Ser-5(P). The basal factor TFIIH can phosphorylate Ser-7 in vitro and is necessary for Ser-7(P) in vivo. Interestingly, deletion of the CTD Ser-5(P) phosphatase Rtr1 leads to an increase in Ser-5(P) but not Ser-7(P).
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Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
---|---|---|---|---|---|---|
General transcription factor TFIIH complex | involved in | phosphorylation of RNA polymerase II C-terminal domain | IDA | ComplexPortal | 2017-04-12 | |
General transcription factor TFIIH complex | enables | RNA polymerase II CTD heptapeptide repeat kinase activity | IDA | ComplexPortal | 2017-04-12 |
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Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
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KIN28 | protein/peptide modification: decreased Reporter: Rpo21p | classical genetics | conditional Allele: kin28-as L83G; bulky residue in the ATP binding pocket mutated to glycine, resulting in an enlarged pocket; analog-sensitive allele | Other | 10 μM 1-NM-PP1 | Details: phosphorylation of serine 5 in the CTD is greatly reduced and serine 7 phosphorylation is reduced to near background levels |
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Regulator | Target | Direction | Regulation Of | Happens During | Method | Evidence |
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KIN28 | RPO21 | protein activity | manually curated | protein kinase assay evidence | ||
TFB5 | RPO21 | protein activity | manually curated | protein kinase assay evidence | ||
TFB1 | RPO21 | protein activity | manually curated | protein kinase assay evidence | ||
TFB3 | RPO21 | protein activity | manually curated | protein kinase assay evidence | ||
RAD3 | RPO21 | protein activity | manually curated | protein kinase assay evidence | ||
SSL2 | RPO21 | protein activity | manually curated | protein kinase assay evidence | ||
SSL1 | RPO21 | protein activity | manually curated | protein kinase assay evidence | ||
TFB2 | RPO21 | protein activity | manually curated | protein kinase assay evidence | ||
CCL1 | RPO21 | protein activity | manually curated | protein kinase assay evidence | ||
TFB4 | RPO21 | protein activity | manually curated | protein kinase assay evidence |