Reference: Prochasson P, et al. (2003)
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Abstract
The SWI/SNF complex is required for the expression of many yeast genes. Previous studies have implicated DNA binding transcription activators in targeting SWI/SNF to UASs and promoters. To determine how activators interact with the complex and to examine the importance of these interactions, relative to other potential targeting mechanisms, for SWI/SNF function, we sought to identify and mutate the activator-interaction domains in the complex. Here we show that the N-terminal domain of Snf5 and the second quarter of Swi1 are sites of activation domain contact. Deletion of both of these domains left the SWI/SNF complex intact but impaired its ability to bind activation domains. Importantly, while deletion of either domain alone had minor phenotypic effect, deletion of both resulted in strong SWI/SNF related phenotypes. Thus, two distinct activator-interaction domains play overlapping roles in the targeting activity of SWI/SNF, which is essential for its function in vivo.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't |
Research Support, U.S. Gov't, P.H.S.
- Authors
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Prochasson P,
Neely KE,
Hassan AH,
Li B,
Workman JL
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- SNF6 | SNF5 | SNF11 | SWI3 | SNF2 | SNF12 | SWI1 | ARP7 | ARP9 | TAF14 | RTT102 | ... Show allSWI/SNF chromatin remodelling complex Show fewer
Gene Ontology Annotations
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
| GCN4 | SWI1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| SNF5 | GCN4 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
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Complement ID |
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