TEF1 / YPR080W Overview

Standard Name
TEF1 1
Systematic Name
eEF1A 6 , EF-1 alpha
Feature Type
ORF , Verified
Translational elongation factor EF-1 alpha; GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides; moonlighting function as an actin binding and bundling protein; association with GTPase Rho1p on the vacuolar membrane may facilitate F-actin remodeling; involved in tRNA re-export from the nucleus 1 2 3 4 5
Name Description
Translation Elongation Factor 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

TEF1 is located on the right arm of chromosome XVI between MRL1 membrane protein and GRS2 glycine-tRNA synthetase; coding sequence is 1377 nucleotides long with 3 silent SNPs; TEF1 has a paralog, TEF2, that arose from the whole genome duplication
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Tef1p is 458 amino acids long and high in abundance; contains 5 GTP-binding elongation factor signature domains; undergoes various post-translational modifications including methylation, phosphorylation, succinylation, and ubiquitinylation on 52 residues scattered throughout the protein
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
533920 +/- 332537


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all TEF1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

GTP- and GDP-binding translation elongation factor; subunit of translation elongation factor 1 complex that binds GTP and aminoacyl-tRNAs, and catalyzes their codon-dependent placement at the A-site of the ribosome; involved in the transport of tRNAs from nucleus to cytoplasm; moonlighting role as an actin filament bundling; associates with the Rho1p GTPase in the vacuole membrane; also localizes to mitochondria and vacuolar membranes

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Overexpression increases cell size, slows growth, and disturbs the actin cytoskeleton
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

The tef1 null mutant is viable; the null mutant of paralog tef2 is viable; the tef1 tef2 double mutant is inviable; interacts physically with proteins involved in translation, interacts genetically with genes involved in tRNA processing and RNA modification

343 total interactions for 271 unique genes

Physical Interactions

  • Affinity Capture-MS: 190
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 19
  • Biochemical Activity: 2
  • Co-crystal Structure: 2
  • Co-fractionation: 6
  • Co-purification: 2
  • PCA: 3
  • Protein-RNA: 1
  • Proximity Label-MS: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 3

Genetic Interactions

  • Dosage Rescue: 12
  • Negative Genetic: 55
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 9
  • Positive Genetic: 9
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Promoter bound by Fhl1p, Fkh2p, and Rap1p; promoter bound by Fhl1p, Gcn5p, Rsf2p, Srb5p, and Tfc8p in response to heat; transcription upregulated by Ifh1p
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.