TEF2 / YBR118W Overview


Standard Name
TEF2 1
Systematic Name
YBR118W
SGD ID
SGD:S000000322
Aliases
eEF1A 7 , EF-1 alpha
Feature Type
ORF , Verified
Description
Translational elongation factor EF-1 alpha; GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides; moonlighting function as an actin binding and bundling protein; association with GTPase Rho1p on the vacuolar membrane may facilitate F-actin remodeling; involved in tRNA re-export from the nucleus; Tef2p-RFP levels increase during replicative aging 1 2 3 4 5 6
Name Description
Translation Elongation Factor 1
Paralog
TEF1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
458
Mol. Weight (Da)
50045.3
Isoelectric Point
9.58
Median Abundance (molecules/cell)
532551 +/- 389013
Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
GTP- and GDP-binding translation elongation factor; subunit of translation elongation factor 1 complex that binds GTP and aminoacyl-tRNAs, and catalyzes their codon-dependent placement at the A-site of the ribosome; involved in the transport of tRNAs from nucleus to cytoplasm; moonlighting role as an actin filament bundling; associates with the Rho1p GTPase in the vacuole membrane; also localizes to mitochondria and vacuolar membranes

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
High-Throughput
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutants are sensitive to TOR inhibitor rapamycin; heterozygous diploid null mutants are heat-tolerant, have unstable chromosomes, and are sensitive to various antibiotics; overexpression increases cell size, interferes with the cell cycle, slows growth, and disturbs the actin cytoskeleton
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The tef2 null mutant is viable; the null mutant of paralog tef1 is viable; the tef2 tef1 double mutant is inviable.

436 total interactions for 345 unique genes

Physical Interactions

  • Affinity Capture-MS: 92
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 11
  • Biochemical Activity: 12
  • Co-crystal Structure: 2
  • Co-fractionation: 4
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 2

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 7
  • Negative Genetic: 223
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 5
  • Positive Genetic: 50
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 4
  • Synthetic Rescue: 2
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
16
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
75
Additional
128
Reviews
21

Resources