ADE13 / YLR359W Overview
- Standard Name
- ADE13 1
- Systematic Name
- YLR359W
- SGD ID
- SGD:S000004351
- Aliases
- BRA1 7 , BRA8 7
- Feature Type
- ORF , Verified
- Description
- Adenylosuccinate lyase; catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency; human ADSL can complement yeast ADE13 null mutant 2 3 4 5
- Name Description
- ADEnine requiring
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 482
- Mol. Weight (Da)
- 54504.0
- Isoelectric Point
- 6.39
- Median Abundance (molecules/cell)
- 32589 +/- 11767
- Half-life (hr)
- 8.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all ADE13 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Adenylosuccinase involved in purine nucleotide and de novo inosine monophosphate (IMP) biosynthetic processes; cellular location is unknown
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in 'de novo' IMP biosynthetic process (IMP, IGI)
- involved in purine nucleotide biosynthetic process (IMP)
Cellular Component
- Manually Curated
- is active in cellular component unknown (ND)
Pathways
- adenosine ribonucleotides de novo biosynthesis
- inosine-5'-phosphate biosynthesis I
- superpathway of purine nucleotides de novo biosynthesis II
- superpathway of adenosine nucleotides de novo biosynthesis II
- superpathway of purine biosynthesis and salvage pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis
- de novo biosynthesis of purine nucleotides
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene in S288C; reduction-of-function mutations result in adenine auxotrophy
Classical Genetics
- null
- overexpression
- reduction of function
- null
- reduction of function
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
86 total interactions for 76 unique genes
Physical Interactions
- Affinity Capture-MS: 29
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 3
- Co-purification: 1
- Proximity Label-MS: 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 2
- Negative Genetic: 22
- Positive Genetic: 10
- Synthetic Haploinsufficiency: 2
- Synthetic Lethality: 4
- Synthetic Rescue: 7
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- ADE13 encodes adenylosuccinate lyase, an enzyme of the purine metabolism and a key component in the regulation system of the purine 'de novo' synthesis pathway. The genes of this pathway are transcriptionally regulated in response to adenine availability by Bas1p and Pho2p. Bas1p, a myb-related transcription factor, binds to promoters of the purine biosynthetic genes, including ADE13, and recruits Pho2p. This interaction stimulates transcription by exposing the activation domain of Bas1p. The binding of Pho2p to Bas1p is enhanced under adenine depletion by two metabolic intermediates, AICAR (5'-phosphoribosyl-5-amino-4-imidazole carboxamide) and SAICAR (succinyl-AICAR), which are a direct product and a substrate, respectively, of the reaction catalyzed by Ade13p. Thus, Ade13p plays a pivotal role in this regulation system by controlling the levels of the two regulatory molecules in the pathway. In addition, AICAR, but not SAICAR, also promotes interaction of Pho2p with Pho4p, another transcription factor that controls transcription of phosphate utilization genes. The human ortholog of ADE13, ADSL, is implicated in a severe metabolic disorder called adenylosuccinate lyase deficiency. ADSL can complement yeast ade13 null mutant.
- Regulators
- 8
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-03-28
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.