ADE13 / YLR359W Overview


Standard Name
ADE13 1
Systematic Name
YLR359W
SGD ID
SGD:S000004351
Aliases
BRA1 7 , BRA8 7
Feature Type
ORF , Verified
Description
Adenylosuccinate lyase; catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency; human ADSL can complement yeast ADE13 null mutant 2 3 4 5
Name Description
ADEnine requiring
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
482
Mol. Weight (Da)
54504.0
Isoelectric Point
6.39
Median Abundance (molecules/cell)
32589 +/- 11767
Half-life (hr)
8.0

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all ADE13 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Adenylosuccinase involved in purine nucleotide and de novo inosine monophosphate (IMP) biosynthetic processes; cellular location is unknown

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene in S288C; reduction-of-function mutations result in adenine auxotrophy
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


88 total interactions for 78 unique genes

Physical Interactions

  • Affinity Capture-MS: 30
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 3
  • Co-purification: 1
  • Proximity Label-MS: 2
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 22
  • Positive Genetic: 10
  • Synthetic Haploinsufficiency: 2
  • Synthetic Lethality: 4
  • Synthetic Rescue: 7
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
ADE13 encodes adenylosuccinate lyase, an enzyme of the purine metabolism and a key component in the regulation system of the purine 'de novo' synthesis pathway. The genes of this pathway are transcriptionally regulated in response to adenine availability by Bas1p and Pho2p. Bas1p, a myb-related transcription factor, binds to promoters of the purine biosynthetic genes, including ADE13, and recruits Pho2p. This interaction stimulates transcription by exposing the activation domain of Bas1p. The binding of Pho2p to Bas1p is enhanced under adenine depletion by two metabolic intermediates, AICAR (5'-phosphoribosyl-5-amino-4-imidazole carboxamide) and SAICAR (succinyl-AICAR), which are a direct product and a substrate, respectively, of the reaction catalyzed by Ade13p. Thus, Ade13p plays a pivotal role in this regulation system by controlling the levels of the two regulatory molecules in the pathway. In addition, AICAR, but not SAICAR, also promotes interaction of Pho2p with Pho4p, another transcription factor that controls transcription of phosphate utilization genes. The human ortholog of ADE13, ADSL, is implicated in a severe metabolic disorder called adenylosuccinate lyase deficiency. ADSL can complement yeast ade13 null mutant.
Regulators
8
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2007-03-28

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
15
Additional
42
Reviews
9

Resources