ADE13 / YLR359W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

ADE13/YLR359W is an essential gene in reference strain S288C; null mutants (ade13-Δ) are inviable on glucose or fructose as a carbon source and exhibit auxotrophy for adenine. Heterozygous null mutants are haploinsufficient, as evidenced by competitive growth assays in a turbidostat. Conditional mutants (ade13-5001) show decreased resistance to micafungin (10μg/mL) but increased resistance to camptothecin (2μg/mL) and acivicin (50μg/mL). Null mutants exhibit increased accumulation of the chemical compound SAICAR (succinylaminoimidazole carboxamide ribose-5’-phosphate). Null mutants have decreased starvation resistance, highlighting the essential role of ADE13 in metabolic and stress response pathways. Overexpression of ADE13 leads to decreased chromosome/plasmid maintenance, characterized by more than a 1.5-fold increase in the loss of a mini-chromosome, thereby indicating chromosome instability (CIN).

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources