REV1 / YOR346W Overview


Standard Name
REV1 1
Systematic Name
YOR346W
SGD ID
SGD:S000005873
Feature Type
ORF , Verified
Description
Bifunctional DNA-directed DNA polymerase/deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress; may be involved in meiosis 2 3 4 5 6
Name Description
REVersionless 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
REV1 is located on the right arm of chromosome XV between replication origin ARS1529 and PYK2 pyruvate kinase; coding sequence is 2958 nucleotides long with 28 SNPs, 12 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Rev1p is 985 amino acids long, very short-lived and low in abundance; phosphorylated on 3 serines; relocalizes from nucleus to cytoplasm upon DNA replication stress
Length (a.a.)
985
Mol. Weight (Da)
112242.7
Isoelectric Point
8.61
Median Abundance (molecules/cell)
1282 +/- 1276
Half-life (hr)
1.1

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all REV1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
DNA-directed DNA polymerase with deoxycytidyl transferase activity; involved in error-prone translesion sythesis; localized to chromatin, replication fork and mitochondrion

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null shows decreased UV resistance, decreased silencing, decreased oxidative stress resistance, decreased mutation frequency, increased accumulation of some amino acids, decreased resistance to cisplatin, oxaliplatin, psoralen, MMS, aflatoxin; null has increased replicative lifespan; heterozygous null is haploinsufficient; overexpression increases oxidative stress resistance
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Rev1p interacts physically with proteins involved in DNA repair; REV1 interacts genetically with genes involved in DNA repair

250 total interactions for 131 unique genes

Physical Interactions

  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 20
  • Co-crystal Structure: 4
  • Co-localization: 1
  • Co-purification: 1
  • Reconstituted Complex: 18
  • Two-hybrid: 15

Genetic Interactions

  • Negative Genetic: 98
  • Phenotypic Enhancement: 23
  • Phenotypic Suppression: 18
  • Positive Genetic: 11
  • Synthetic Growth Defect: 22
  • Synthetic Lethality: 8
  • Synthetic Rescue: 3
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
REV1 promoter is bound by Uga3p in response to heat
Regulators
1
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2010-02-02

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
113
Additional
101
Reviews
58

Resources