ADH1 / YOL086C Overview


Standard Name
ADH1 1
Systematic Name
YOL086C
SGD ID
SGD:S000005446
Aliases
ADC1
Feature Type
ORF , Verified
Description
Alcohol dehydrogenase; fermentative isozyme active as homo- or heterotetramers; required for the reduction of acetaldehyde to ethanol, the last step in the glycolytic pathway; ADH1 has a paralog, ADH5, that arose from the whole genome duplication 2 3 4 5
Name Description
Alcohol DeHydrogenase
Paralog
ADH5 5
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
ADH1 is located on the left arm of chromosome XV between MHF1 component of the heterotetrameric MHF histone-fold complex and uncharacterized gene YOL085C; coding sequence is 1047 nucleotides long with 19 SNPs, 16 of which are clustered between nucleotides 366 and 455; of the 19 SNPs, 13 are silent; ADH1 has a paralog, ADH5, that arose from the whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Adh1p is 348 amino acids long, somewhat short-lived and highly abundant; contains 2 alcohol dehydrogenase GroES-like domains; undergoes various post-translational polymorphisms, including acetylation, phosphorylation, succinylation, sumoylation, and ubiquitinylation on 41 residues
Length (a.a.)
348
Mol. Weight (Da)
36845.5
Isoelectric Point
6.67
Median Abundance (molecules/cell)
103727 +/- 63773
Half-life (hr)
6.1

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all ADH1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Alcohol dehydrogenase required for the reduction of acetaldehyde to ethanol during fermentation; also has methylglyoxal reductase activity, and is involved in NADH oxidation and the production of alcohol from amino acid breakdown via the Ehrlich pathway

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant cells are large and show decreased fermentative growth rate, sensitivity to oxidative stress, antifungal drugs, and other chemicals
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The adh1 null mutant is viable; the null mutant of paralog adh5 is viable; the adh1 adh5 double mutant displays a growth defect; interacts physically with proteins involved in translation and transcription; interacts genetically with genes involved in transcription

310 total interactions for 265 unique genes

Physical Interactions

  • Affinity Capture-MS: 137
  • Affinity Capture-RNA: 9
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • Co-fractionation: 16
  • Co-localization: 1
  • Co-purification: 2
  • PCA: 4
  • Protein-RNA: 6
  • Proximity Label-MS: 2
  • Two-hybrid: 4

Genetic Interactions

  • Dosage Rescue: 1
  • Negative Genetic: 82
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 4
  • Positive Genetic: 19
  • Synthetic Growth Defect: 5
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
Transcription upregulated by Msn2/Msn4p, and by Znf1p during glycolysis; promoter bound by 13 different transcription factors in response to heat
Regulators
27
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2008-08-19

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
197
Additional
515
Reviews
99

Resources