BRE5 / YNR051C Overview

Standard Name
BRE5 1
Systematic Name
Feature Type
ORF , Verified
Ubiquitin protease cofactor; forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the ER and Golgi compartments, deubiquitinating COPII and COPI vesicle coat constituents, Sec23p and Sec27p; involved along with Ubp3p in the steady-state retention of Golgi membrane proteins, such as glycosyltransferases; null is sensitive to brefeldin A 1 2 3 4
Name Description
BREfeldin A sensitivity 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
7140 +/- 1929
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all BRE5 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Protein involved in deubiquitination, ribophagy, regulation of ER to Golgi vesicle-mediated transport and retrograde vesicle-mediated transport (golgi to ER); localizes to the cytoplasmic mRNA processing body and has been shown to bind to mRNA in large scale studies

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant is heat sensitive, displays decreased competitive fitness on numerous growth media, has a decreased ability to utilize glucose as carbon source, and allantoin or glutamine as nitrogen sources; null mutant has abnormal nuclear morphology, filamentous and invasive growth defects, decreased stress resistance and a decreased ability to undergo ribophagy in response to nitrogen starvation; null mutant displays decreased resistance to cell wall stressors, antifungal agents and DNA synthesis inhibitors; null mutant has numerous alterations in the amino acid metabolome; null mutant has an increased replicative lifespan; null mutant has a defect in retrograde transport from the Golgi to the ER; homozygous diploid null displays a defect in meiosis with reduced gene conversion, and abnormal sporulation with an altered DL:LL dityrosine ratio; heterozygous diploid null mutant is haploproficient
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

2345 total interactions for 1286 unique genes

Physical Interactions

  • Affinity Capture-MS: 519
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 10
  • Co-crystal Structure: 2
  • Co-purification: 1
  • PCA: 5
  • Proximity Label-MS: 4
  • Reconstituted Complex: 3
  • Two-hybrid: 3

Genetic Interactions

  • Dosage Growth Defect: 2
  • Dosage Rescue: 5
  • Negative Genetic: 1327
  • Phenotypic Enhancement: 30
  • Phenotypic Suppression: 38
  • Positive Genetic: 290
  • Synthetic Growth Defect: 68
  • Synthetic Lethality: 23
  • Synthetic Rescue: 7
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.