ACC1 / YNR016C Overview
- Standard Name
- ACC1 1
- Systematic Name
- YNR016C
- SGD ID
- SGD:S000005299
- Aliases
- MTR7 11 , FAS3 7 , ABP2 12
- Feature Type
- ORF , Verified
- Description
- Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availability of acetyl-CoA; rate-limiting step for de novo biosynthesis of long-chain fatty acids; translational regulation in response to nutrients and cell cycle stage depends on its upstream ORF; ACC1 has a paralog, HFA1, that arose from the whole genome duplication 1 2 3 4 5
- Name Description
- Acetyl-CoA Carboxylase 1
- Paralog
- HFA1 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- ACC1 is located on the right arm of chromosome XIV near the centromere between SMM1 dihydrouridine synthase and mitochondrial protein TIM23; coding sequence is 6702 nucleotides long with a uORF at -340..-329, and 18 SNPs in the coding region, one of which leads to a Ser/Ala amino acid polymorphism at residue 1257; ACC1 has paralog HFA1 that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Acc1p is 2233 amino acids long, longer-lived, highly abundant; acetylated on 13 lysines, ubiquitinylated on 34 lysines, succinylated on K1464 and K2137, phosphorylated on 41 residues
- Length (a.a.)
- 2233
- Mol. Weight (Da)
- 250318.7
- Isoelectric Point
- 6.21
- Median Abundance (molecules/cell)
- 29049 +/- 13718
- Half-life (hr)
- 11.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ACC1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Acetyl-CoA- and biotin carboxylase involved in long-chain fatty acid biosynthesis; plays a role in protein import into nucleus; localizes to cytosol, mitochondria, and endoplasmic reticulum membrane
View computational annotations
Molecular Function
- Manually Curated
- enables acetyl-CoA carboxylase activity (IMP)
- enables biotin carboxylase activity (IMP)
Biological Process
- Manually Curated
- involved in long-chain fatty acid biosynthetic process (IMP)
- involved in protein import into nucleus (IMP)
Cellular Component
- Manually Curated
- located in cytosol (HDA)
- located in endoplasmic reticulum membrane (IDA)
- located in mitochondrion (HDA)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutants accumulate poly(A) RNA in the nucleus; overexpression leads to increased lipid accumulation; reduction of function results in chromosome instability, decreased competitive fitness, heat sensitivity
Classical Genetics
- conditional
- unspecified
- overexpression
- activation
- reduction of function
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Acc1p interacts physically with proteins involved in translation; ACC1 interacts genetically with genes involved in chromosome segregation, mitotic cell cycle, and DNA repair; the acc1 null mutant is inviable; the null mutant of paralog hfa1 is viable; the acc1 hfa1 double mutant has not been annotated for phenotype.
356 total interactions for 326 unique genes
Physical Interactions
- Affinity Capture-MS: 107
- Affinity Capture-RNA: 8
- Co-crystal Structure: 1
- Co-localization: 1
- Co-purification: 1
- PCA: 18
- Protein-RNA: 1
- Proximity Label-MS: 3
- Two-hybrid: 10
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 153
- Phenotypic Enhancement: 5
- Phenotypic Suppression: 6
- Positive Genetic: 28
- Synthetic Growth Defect: 2
- Synthetic Lethality: 1
- Synthetic Rescue: 10
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 6
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 1999-11-02
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.