GAL80 / YML051W Overview


Standard Name
GAL80 1
Systematic Name
YML051W
SGD ID
SGD:S000004515
Feature Type
ORF , Verified
Description
Transcriptional regulator involved in the repression of GAL genes; involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding 2
Name Description
GALactose metabolism
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
GAL80 is located on the left arm of chromosome XIII between membrane protein SUR7 and 2Fe-2S mitochondrial protein AIM32; coding sequence is 1308 nucleotides long with 9 SNPs, only one of which causes an amino acid polymorphism, Glu/Asp at residue 101
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Gal80p is 435 amino acids long, low in abundance; contains 2 NAD(P)-binding domains; phosphorylated on 4 residues
Length (a.a.)
435
Mol. Weight (Da)
48324.7
Isoelectric Point
8.17
Median Abundance (molecules/cell)
1153 +/- 749

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all GAL80 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Transcription factor-binding protein involved in galactose metabolism; negatively regulates Gal4p transcription factor activity; localizes to the nucleus and cytoplasm

View computational annotations

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant shows decreased growth rate of newly germinated spores, small defect in vacuolar fragmentation, decreased growth in the absence of galactose, decreased chronological lifespan, sensitivity to UV, formamide, and heat
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Gal80p interacts physically with proteins involved in transcription; GAL80 interacts genetically with genes involved in transcription

339 total interactions for 235 unique genes

Physical Interactions

  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 14
  • Co-crystal Structure: 3
  • Co-localization: 1
  • Co-purification: 3
  • FRET: 1
  • PCA: 11
  • Protein-peptide: 5
  • Reconstituted Complex: 26
  • Two-hybrid: 40

Genetic Interactions

  • Dosage Growth Defect: 3
  • Dosage Lethality: 2
  • Dosage Rescue: 2
  • Negative Genetic: 157
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 3
  • Positive Genetic: 33
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1
  • Synthetic Rescue: 13
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
GAL80 promoter is bound by Fkh1p, Fkh2p, and Gal4p; GAL80 promoter is bound by Gcn5p and Yap1p in response to heat
Regulators
5
Targets
4
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2006-10-04

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
106
Additional
184
Reviews
46

Resources