TSA1 / YML028W Overview


Standard Name
TSA1 1
Systematic Name
YML028W
SGD ID
SGD:S000004490
Aliases
cTPxI 13 , ZRG14 14 , TPX1
Feature Type
ORF , Verified
Description
Thioredoxin peroxidase; acts as both ribosome-associated and free cytoplasmic antioxidant; self-associates to form a HMW chaperone complex under oxidative stress; chaperone activity essential for growth in zinc deficiency; hydrogen peroxide receptor and signal transducer in a light sensing pathway; required for telomere length maintenance; binds and modulates Cdc19p activity; protein abundance increases and forms cytoplasmic foci during DNA replication stress 1 2 3 4 5 6 7 8 9 10 11 12
Name Description
Thiol-Specific Antioxidant 1
Paralog
TSA2
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
TSA1 has a paralog, TSA2, that arose from the whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Five homodimers of Tsa1p can assemble into a toroid-shaped decamer, with the active sites in the enzyme being shared between individual dimers in the decamer; oligomerization is promoted by aromatic residues at the decamer-building interface, substitution of which can destabilize the decamer
Length (a.a.)
196
Mol. Weight (Da)
21583.8
Isoelectric Point
4.77
Median Abundance (molecules/cell)
103335 +/- 66024
Half-life (hr)
10.9

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all TSA1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Thioredoxin peroxidase; involved in control of cellular redox homeostasis and response to oxidative stress; localized on polysomes and in cytoplasm

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant is hypersensitive to oxidative stress and shows decreased anaerobic growth rate, increased mutation frequency, sensitivity to DNA-damaging factors, such as UV, 4-NQO and hydroxyurea
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The tsa1 null mutant is viable; the null mutant of paralog tsa2 is viable; the tsa1 tsa2 double mutant displays a synthetic growth defect.

1054 total interactions for 647 unique genes

Physical Interactions

  • Affinity Capture-MS: 114
  • Affinity Capture-RNA: 18
  • Affinity Capture-Western: 14
  • Biochemical Activity: 2
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-localization: 2
  • Co-purification: 6
  • Proximity Label-MS: 3
  • Reconstituted Complex: 12
  • Two-hybrid: 4

Genetic Interactions

  • Dosage Growth Defect: 2
  • Dosage Rescue: 3
  • Negative Genetic: 434
  • Phenotypic Enhancement: 20
  • Phenotypic Suppression: 13
  • Positive Genetic: 62
  • Synthetic Growth Defect: 243
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 89
  • Synthetic Rescue: 9
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
14
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
128
Additional
142
Reviews
37

Resources