SRS2 / YJL092W Overview


Standard Name
SRS2 1
Systematic Name
YJL092W
SGD ID
SGD:S000003628
Aliases
HPR5 14 , RADH 14 , RADH1
Feature Type
ORF , Verified
Description
DNA helicase and DNA-dependent ATPase; role in DNA repair and checkpoint recovery, in the proper timing of commitment to meiotic recombination and the Meiosis I to II transition; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; stimulates Mus81p-Mms4p endonuclease activity independent of catalytic activity; ATPase and ssDNA translocating motor activities inhibited by Dmc1p; functional homolog of human RTEL1 2 3 4 5 6 7 8 9 10 11 12 13
Name Description
Suppressor of Rad Six 1
Comparative Info
Integrated model organism details available at the Alliance of Genome Resources website
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
1174
Mol. Weight (Da)
134321.7
Isoelectric Point
9.21
Median Abundance (molecules/cell)
673 +/- 579
Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
DNA helicase involved in double-strand break repair, DNA duplex unwinding and disassembling DNA-protein complexes; localized to the nucleus

View computational annotations

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant shows chromosome instability, increased mitotic recombination and UV sensitivity; in systematic studies null mutants are sensitive to DNA damaging factors, such as gamma rays, X rays, camptothecin, cisplating, MMS, hydroxyurea; homozygous null mutant diploids show reduced spore germination; overexpression causes delay in S phase of the cell cycle
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


1303 total interactions for 565 unique genes

Physical Interactions

  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 12
  • Biochemical Activity: 4
  • Co-crystal Structure: 2
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 29
  • Two-hybrid: 103

Genetic Interactions

  • Dosage Lethality: 331
  • Dosage Rescue: 42
  • Negative Genetic: 246
  • Phenotypic Enhancement: 24
  • Phenotypic Suppression: 39
  • Positive Genetic: 34
  • Synthetic Growth Defect: 189
  • Synthetic Lethality: 123
  • Synthetic Rescue: 103
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
7
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
153
Additional
99
Reviews
61

Resources