Protein Help

SPT6 / YGR116W Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.


Aliases
CRE2 9 , SSN20 10
Protein Product
chromatin-remodeling histone chaperone SPT6
Feature Type
ORF , Verified
Summary
Spt6p is 1451 amino acids long, of average half-life, moderate in abundance; ubiquitinylated on K1355, acetylated on 4 lysines, phosphorylated on 18 residues

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.


Downloading... 1.00 MB

Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.


Protein Half Life

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ExperimentResultReference
half-life9.8 hrChristiano R, et al. (2014)

Protein Abundance

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Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
6938SDuntreatedconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
7192SD2 mM 1,4-dithiothreitol2 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
8504SD1 mM hydrogen peroxide1 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
6939SD minus nitrogencellular response to nitrogen starvation15 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
7230SDuntreatedconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
Showing 1 to 5 of 28 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.


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Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


spt6-1004 | spt6-14 | spt6-140 | spt6-50 | spt6-AID | spt6-F249K | spt6-S1284A | ... Show all

View all SPT6 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.


1 MEETGDSKLV PRDEEEIVND NDETKAPSEE EEGEDVFDSS EEDEDIDEDE DEARKVQEGF
61 IVNDDDENED PGTSISKKRR KHKRREREED DRLSEDDLDL LMENAGVERT KASSSSGKFK
121 RLKRVGDEGN AAESESDNVA ASRQDSTSKL EDFFSEDEEE EESGLRNGRN NEYGRDEEDH
181 ENRNRTADKG GILDELDDFI EDDEFSDEDD ETRQRRIQEK KLLREQSIKQ PTQITGLSSD
241 KIDEMYDIFG DGHDYDWALE IENEELENGN DNNEAEEEEI DEETGAIKST KKKISLQDIY
301 DLEDLKKNLM TEGDMKIRKT DIPERYQELR AGITDYGNMS SEDQELERNW IAEKISVDKN
361 FDANYDLTEF KEAIGNAIKF ITKENLEVPF IYAYRRNYIS SREKDGFLLT EDDLWDIVSL
421 DIEFHSLVNK KDYVQRFYAE LHIDDPIVTE YFKNQNTASI AELNSLQDIY DYLEFKYANE
481 INEMFINHTG KTGKKHLKNS SYEKFKASPL YQAVSDIGIS AEDVGENISS QHQIHPPVDH
541 PSSKPVEVIE SILNANSGDL QVFTSNTKLA IDTVQKYYSL ELSKNTKIRE KVRSDFSKYY
601 LADVVLTAKG KKEIQKGSLY EDIKYAINRT PMHFRRDPDV FLKMVEAESL NLLSVKLHMS
661 SQAQYIEHLF QIALETTNTS DIAIEWNNFR KLAFNQAMDK IFQDISQEVK DNLTKNCQKL
721 VAKTVRHKFM TKLDQAPFIP NVRDPKIPKI LSLTCGQGRF GADAIIAVYV NRKGDFIRDY
781 KIVDNPFDKT NPEKFEDTLD NIIQSCQPNA IGINGPNPKT QKFYKRLQEV LHKKQIVDSR
841 GHTIPIIYVE DEVAIRYQNS ERAAQEFPNK PPLVKYCIAL ARYMHSPLLE YANLTSEEVR
901 SLSIHPHQNL LSSEQLSWAL ETAFVDIVNL VSVEVNKATD NNYYASALKY ISGFGKRKAI
961 DFLQSLQRLN EPLLARQQLI THNILHKTIF MNSAGFLYIS WNEKRQKYED LEHDQLDSTR
1021 IHPEDYHLAT KVAADALEYD PDTIAEKEEQ GTMSEFIELL REDPDRRAKL ESLNLESYAE
1081 ELEKNTGLRK LNNLNTIVLE LLDGFEELRN DFHPLQGDEI FQSLTGESEK TFFKGSIIPV
1141 RVERFWHNDI ICTTNSEVEC VVNAQRHAGA QLRRPANEIY EIGKTYPAKV IYIDYANITA
1201 EVSLLDHDVK QQYVPISYSK DPSIWDLKQE LEDAEEERKL MMAEARAKRT HRVINHPYYF
1261 PFNGRQAEDY LRSKERGEFV IRQSSRGDDH LVITWKLDKD LFQHIDIQEL EKENPLALGK
1321 VLIVDNQKYN DLDQIIVEYL QNKVRLLNEM TSSEKFKSGT KKDVVKFIED YSRVNPNKSV
1381 YYFSLNHDNP GWFYLMFKIN ANSKLYTWNV KLTNTGYFLV NYNYPSVIQL CNGFKTLLKS
1441 NSSKNRMNNY R*

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

94 entries for 29 sites

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SiteModificationModifierReference
S7phosphorylated residueZhou X, et al. (2021) PMID: 33481703
T24phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
S28phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
S28phosphorylated residueAlbuquerque CP, et al. (2008) PMID: 18407956
S39phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
S40phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
S74phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
S74phosphorylated residueZhou X, et al. (2021) PMID: 33481703
S74phosphorylated residueSwaney DL, et al. (2013) PMID: 23749301
S74phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
Showing 1 to 10 of 94 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A775.31
C70.48
D1238.48
E15410.61
F584.00
G563.86
H332.27
I1037.10
K1167.99
L1238.48
M201.38
N996.82
P453.10
Q594.07
R745.10
S996.82
T634.34
V704.82
W110.76
Y614.20

Physical Details

Length (a.a): 1451
Molecular Weight (Da): 168205.4
Isoelectric Point (pl): 4.63
Formula: C7427H11501N2024O2387S27
Aliphatic Index: 74.51
Instability Index: 47.15

Coding Region Translation Calculations

Codon Bias: 0.18
Codon Adaptation Index: 0.22
Frequence of Optimal Codons: 0.55
Hydropathicity of Protein: -0.78
Aromaticity Score: 0.09

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 151765.0
NO Cys residues appear as half cystines: 151390.0

Atomic Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

48 entries for 10 sources


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External IDSource
orf19.7136CGD
2630DIP
CAA97127.1GenBank/EMBL/DDBJ
CAA97124.1GenBank/EMBL/DDBJ
AAA35086.1GenBank/EMBL/DDBJ
6321552GenBank/EMBL/DDBJ
Z72899GenBank/EMBL/DDBJ
Z72902GenBank/EMBL/DDBJ
327200640GenBank/EMBL/DDBJ
327200639GenBank/EMBL/DDBJ
Showing 1 to 10 of 48 entries

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