Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
7 entries for 4 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|---|
| SPT6 | cell cycle progression in S phase: delayed | classical genetics | reduction of function Allele: spt6-50 K1274stop; premature termination of the C-terminal domain; tandem SH2 domains deleted | S288C | Treatment: alpha-factor block release Details: delayed DNA synthesis with an increase in the number of cells with DNA content between 1N and 2N | Miller CLW and Winston F (2023) PMID:36924499 | |
| SPT6 | mitotic recombination: increased | classical genetics | reduction of function Allele: spt6-50 K1274stop; premature termination of the C-terminal domain; tandem SH2 domains deleted | S288C | Details: 32-fold increase relative to wt, using an inverted repeat recombination assay | Miller CLW and Winston F (2023) PMID:36924499 | |
| SPT6 | protein/peptide accumulation: increased Reporter: Rad52-YFP | classical genetics | reduction of function Allele: spt6-50 K1274stop; premature termination of the C-terminal domain; tandem SH2 domains deleted | S288C | Phase: G2/M Details: large increase in Rad52-YFP foci | Miller CLW and Winston F (2023) PMID:36924499 | |
| SPT6 | resistance to chemicals: decreased | classical genetics | reduction of function Allele: spt6-50 K1274stop; premature termination of the C-terminal domain; tandem SH2 domains deleted | S288C | 100 mM hydroxyurea | Details: hypersensitive; partial growth | Miller CLW and Winston F (2023) PMID:36924499 |
| SPT6 | resistance to chemicals: decreased | classical genetics | reduction of function Allele: spt6-50 | S288C | 50 ug/ml 6-azauracil | Hartzog GA, et al. (1998) PMID:9450930 | |
| SPT6 | resistance to chemicals: decreased | classical genetics | reduction of function Allele: spt6-50 K1274stop; premature termination of the C-terminal domain; tandem SH2 domains deleted | S288C | 5 μg/mL phleomycin | Details: hypersensitive; no detectable growth | Miller CLW and Winston F (2023) PMID:36924499 |
| SPT6 | resistance to chemicals: decreased | classical genetics | reduction of function Allele: spt6-50 K1274stop; premature termination of the C-terminal domain; tandem SH2 domains deleted | S288C | 0.024% methyl methanesulfonate | Details: hypersensitive; no detectable growth | Miller CLW and Winston F (2023) PMID:36924499 |
This diagram displays up to the top 30 positive interactions (green), negative interactions (orange), and phenotypes (blue) that are shared between the given allele (black) and other alleles (purple). The shared interactions or phenotypes can be visualized separately using the radio button at the bottom of the graph.
List of references used specifically to curate the information in Overview section.
Addgene Plasmids | DNASU Plasmids | PlasmID | Thermo Scientific | YGRC
dHITS | FitDB | HIPHOP Chemogenomics | HIP HOP Profiles | PhenoM | PROPHECY | SCMD | ScreenTroll | TheCellVision | Yeast Phenome
BioGRID | CYC2008 | DIP | GeneMANIA | IMP | InterologFinder | ModelArchive | TheCellMap