Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.
18 entries for 11 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|
| cell cycle progression in S phase: abnormal | large-scale survey | repressible | S288C | Details: accumulation of cells with a DNA content between 1C and 2C indicative of an S phase delay | Yu L, et al. (2006) PMID:16943325 | |
| chromosome/plasmid maintenance: decreased | large-scale survey | repressible | S288C | 10 ug/ml doxycycline | Details: Tet-regulated essential ORF strain collection (doxycycline represses expression)|; increased spontaneous DNA damage as measured by an increase of Ddc2 foci relative to wild-type | Cheng E, et al. (2012) PMID:22673806 |
| competitive fitness: decreased | competitive growth fitness profiling of essential genes using hypomorphic DAmP alleles | reduction of function | S288C | Media: minimal medium Details: Relative fitness score: 0.976 | Breslow DK, et al. (2008) PMID:18622397 | |
| haploinsufficient | heterozygous diploid, systematic mutation set | null Allele: erg26-Δ | S288C | Ohnuki S and Ohya Y (2018) PMID:29768403 | ||
| inviable | systematic mutation set | null Allele: erg26-Δ | S288C | Giaever G, et al. (2002) PMID:12140549 | ||
| mitochondrial morphology: abnormal | systematic mutation set | repressible | S288C | 10 ug/ml doxycycline | Details: Tet-regulated essential ORF strain collection; doxycycline represses expression | Altmann K and Westermann B (2005) PMID:16135527 |
| protein/peptide distribution: decreased Reporter: Pmp2GFP-KKTN | classical genetics | repressible | S288C | Details: Reporter localizes to the ER in wildtype | Michelsen K, et al. (2007) PMID:17954604 | |
| protein/peptide distribution: decreased Reporter: Pmp2GFP-LRKR last 36 residues of the mammalian potassium channel Kir6.2 fused to Pmp2p | large-scale survey | repressible | S288C | Details: Reporter localizes to the ER in wildtype | Michelsen K, et al. (2007) PMID:17954604 | |
| resistance to chemicals: decreased | heterozygous diploid, systematic mutation set | null Allele: erg26-Δ | S288C | ascomycin | Hoepfner D, et al. (2014) PMID:24360837 | |
| resistance to chemicals: decreased | heterozygous diploid, competitive growth | null Allele: erg26-Δ | S288C | 4 uM FR171456 | Details: shows the most significant hypersensitivity in haploinsufficiency profiling (HIP) | Helliwell SB, et al. (2015) PMID:26456460 |
This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).
Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.
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