DOT1 / YDR440W Overview


Standard Name
DOT1 1
Systematic Name
YDR440W
SGD ID
SGD:S000002848
Aliases
KMT4 7 , PCH1 3
Feature Type
ORF , Verified
Description
Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response; single DOT1 gene produces two Dot1p isoforms from alternative translation start sites as a result of leaky scanning by the ribosome 2 3 4 5 6
Name Description
Disruptor Of Telomeric silencing 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
DOT1/YDR440W is located on the right arm of chromosome IV toward the telomere between LRS4 nucleolar protein and APT2 adenine phosphoribosyltransferase; coding sequence is 1749 nucleotides long with 15 SNPs, 7 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Dot1p is 582 amino acids long, somewhat short-lived, low in abundance; contains disordered regions in N-terminus; ubiquitinylated on K43 and K50, acetylated on 10 residues, phosphorylated on 15 residues
Length (a.a.)
582
Mol. Weight (Da)
66219.2
Isoelectric Point
9.46
Median Abundance (molecules/cell)
1310 +/- 850
Half-life (hr)
6.2

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all DOT1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Histone methyltransferase, specific to H3-K79; involved in DNA damage checkpoint, recombinational and nucleotide-excision repair and chromatin silencing at telomeres; localized to the nucleus

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant exhibits sensitivity to gamma rays but resistance to X rays and MMS; null mutant shows decreased silencing and has telomeres of heterogeneous length; overexpression also causes decreased silencing; in large-scale studies, null mutant has shortened chronological lifespan, increased resistance to heat, and altered resistance to various chemicals
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast DOT1 is homologous to human DOT1L, and has been used to study deregulation of histone H3K79 methyltransferase activity in human leukemias

Manually Curated

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Dot1p interacts physically with proteins involved in chromatin organization and DNA repair; DOT1 interacts genetically with genes involved in DNA repair and transcription

469 total interactions for 313 unique genes

Physical Interactions

  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 6
  • Biochemical Activity: 18
  • Co-crystal Structure: 3
  • Co-localization: 3
  • Far Western: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Growth Defect: 5
  • Dosage Rescue: 2
  • Negative Genetic: 275
  • Phenotypic Enhancement: 23
  • Phenotypic Suppression: 20
  • Positive Genetic: 13
  • Synthetic Growth Defect: 42
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 6
  • Synthetic Rescue: 28
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
DOT1 promoter is bound by Cin5p in response to heat; DOT1 transcription is regulated by Pdr1p
Regulators
2
Targets
4
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2024-08-19

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
92
Additional
92
Reviews
56

Resources