NPL3 / YDR432W Overview

Standard Name
NPL3 1
Systematic Name
NAB1 12 , MTR13 13 , MTS1 14 , NOP3 15
Feature Type
ORF , Verified
RNA-binding protein; promotes elongation, regulates termination, and is involved in nuclear export of poly(A) mRNA; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ligase Bre1p, linking histone ubiquitination to mRNA processing; binds TERRA and stabilizes R-loops at short telomeres; phosphorylation by Sky1p and interaction with Mtr10p promotes shuttling and mRNA dissociation; protein abundance increases in response to DNA replication stress 2 3 4 5 6 7 8 9 10 11
Name Description
Nuclear Protein Localization 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
34936 +/- 26291


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all NPL3 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

RNA-binding protein involved in pre-mRNA splicing and mRNA export from the nucleus; regulates RNA pol II transcription elongation and termination; implicated in translation termination and repression of translational initiation; shuttles between the cytoplasm and nucleus

View computational annotations

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene in S288C, reported essential in Sigma1278b; null mutant is sensitive to elevated temperatures, metals and multiple drugs, including rapamycin, paromomycin, tacrolimus; in systematic studies homozygous null mutant diploid shows slow growth, decreased fermentative growth, inositol auxotrophy, decreased innate thermotolerance, abnormal budding pattern and elevated sensitivity to multiple drugs, including doxorubicin, echinocandin, fenpropimorph, hygromycin B
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

1502 total interactions for 1172 unique genes

Physical Interactions

  • Affinity Capture-MS: 166
  • Affinity Capture-RNA: 14
  • Affinity Capture-Western: 56
  • Biochemical Activity: 20
  • Co-localization: 3
  • Co-purification: 1
  • Far Western: 1
  • PCA: 1
  • Protein-peptide: 6
  • Protein-RNA: 3
  • Reconstituted Complex: 20
  • Two-hybrid: 20

Genetic Interactions

  • Dosage Lethality: 2
  • Dosage Rescue: 9
  • Negative Genetic: 71
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 17
  • Positive Genetic: 18
  • Synthetic Growth Defect: 376
  • Synthetic Lethality: 39
  • Synthetic Rescue: 656
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.