SAC6 / YDR129C Overview


Standard Name
SAC6 1 2
Systematic Name
YDR129C
SGD ID
SGD:S000002536
Aliases
ABP67 11
Feature Type
ORF , Verified
Description
Fimbrin, actin-bundling protein; cooperates with Scp1p in organization and maintenance of the actin cytoskeleton; phosphorylated by Cdc28p/Clb2p in metaphase on T103, to regulate conformation, and modulate actin filament binding affinity and actin cable dynamics; relocalizes from the plasma membrane to the cytoplasm upon DNA replication stress; human homologs PLS3 and LCP1 implicated in spinocerebellar ataxia type 2 (SCA2) can each complement yeast null mutant 3 4 5 6 8 9 10
Name Description
Suppressor of ACtin 7
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
642
Mol. Weight (Da)
71755.3
Isoelectric Point
5.13
Median Abundance (molecules/cell)
25994 +/- 5216

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all SAC6 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Actin filament binding protein involved in actin filament organization and formin-nucleated actin cable assembly; localizes to bud tips and mating projection tips in high-throughput studies

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
High-Throughput
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant grows slowly, fails to localize actin patches preferentially to growing buds, and has a severe endocytosis defect; null mutant is sensitive to metal ions and latrunculin A; in large-scale studies, null mutant has reduced competitive fitness in several different media, exhibits a respiratory growth defect, and is sensitive to heat, starvation, acid pH, and K. lactis zymocin; null mutant has increased chitin deposition and is resistant to K28 killer toxin; null mutant is sensitive to a variety of chemicals
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast SAC6 is homologous to human LCP1 and PLS3, and has been used to study Huntington's disease

Manually Curated

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


392 total interactions for 303 unique genes

Physical Interactions

  • Affinity Capture-MS: 60
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 3
  • Biochemical Activity: 1
  • Co-fractionation: 3
  • Co-localization: 1
  • Co-purification: 2
  • PCA: 7
  • Proximity Label-MS: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 2

Genetic Interactions

  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 173
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 5
  • Positive Genetic: 24
  • Synthetic Growth Defect: 30
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 43
  • Synthetic Rescue: 13
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
3
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
54
Additional
67
Reviews
29

Resources