Phenotype Help

SLD2 / YKL108W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.



Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

24 entries for 14 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
cell cycle progression in S phase: abnormal
large-scale surveyrepressibleS288CDetails: accumulation of cells with a DNA content between 1C and 2C indicative of an S phase delay
Yu L, et al. (2006) PMID:16943325
cell cycle progression in S phase: arrested
classical geneticsconditionalOtherTemperature: elevated temperature, 37 °C
Details: arrest as large budded cells with single nucleus and DNA content between 1N and 2N, indicating defect in DNA replication
Wang H and Elledge SJ (1999) PMID:10097122
cell cycle progression through the G1/S phase transition: abnormal
classical geneticsreduction of function
Allele: sld2-m1,4

defective in binding Mcm2p-7p

OtherDetails: DNA replication does not occur upon release from induced G1 arrest; indicates requirement for entry into S phase
Bruck I and Kaplan DL (2014) PMID:24307213
chromosome/plasmid maintenance: abnormal
systematic mutation set

hypomorphic DAmP allele in which an insertion in the 3'UTR reduces mRNA stability

reduction of functionS288CAssay: gross-chromosomal rearrangement (GCR) assay
Details: indicative of chromosome instability (CIN); metascore strong (single hit)
Stirling PC, et al. (2011) PMID:21552543
chromosome/plasmid maintenance: decreased
systematic mutation setoverexpressionOtherAssay: a-like faker (ALF) assay
Details: dosage chromosome instability (dCIN) involving chromosome loss, rearrangement and/or gene conversion events
Duffy S, et al. (2016) PMID:27551064
chromosome/plasmid maintenance: decreased
systematic mutation setoverexpressionOtherAssay: chromosome transmission fidelity (CTF) assay
Details: dosage chromosome instability (dCIN) involving chromosome loss
Duffy S, et al. (2016) PMID:27551064
chromosome/plasmid maintenance: decreased
heterozygous diploid, systematic mutation setnull
Allele: sld2-Δ
S288CAssay: diploid bimater (BiM) assay
Details: haploinsufficiency results in chromosome instability (CIN); scored >= 2 standard deviations above the mean
Choy JS, et al. (2013) PMID:23825022
competitive fitness: decreased
competitive growth

fitness profiling of essential genes using hypomorphic DAmP alleles

reduction of functionS288CMedia: minimal medium
Details: Relative fitness score: 0.996
Breslow DK, et al. (2008) PMID:18622397
haploproficient
heterozygous diploid, competitive growth

genome-wide fitness profiling

null
Allele: sld2-Δ
S288CMedia: turbidostat growth in FPM medium
Details: Relative growth score: 0.0182
Pir P, et al. (2012) PMID:22244311
heat sensitivity: increased
classical geneticsconditional
Allele: sld2-6
OtherTemperature: elevated temperature, 34 °C
Kamimura Y, et al. (1998) PMID:9742127
Showing 1 to 10 of 24 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


Reset

Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.


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