RGT1 / YKL038W Overview


Standard Name
RGT1 1
Systematic Name
YKL038W
SGD ID
SGD:S000001521
Feature Type
ORF , Verified
Description
Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; recruits Tup1p/Cyc8p to target gene promoters; RGT1 has a paralog, EDS1, that arose from the whole genome duplication 2 3 4 5
Name Description
Restores Glucose Transport 1
Paralog
EDS1 4
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
RGT1 has a paralog, EDS1, that arose from the whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
1170
Mol. Weight (Da)
128244.9
Isoelectric Point
8.0
Median Abundance (molecules/cell)
520 +/- 503

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all RGT1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
DNA-binding transcription factor, acts as both an activator and a repressor; involved in the regulation of glucose metabolism; localizes to the nucleus

View computational annotations

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The rgt1 null mutant is viable; the null mutant of paralog eds1 is viable; the rgt1 eds1 double mutant has not been annotated for phenotype.

172 total interactions for 132 unique genes

Physical Interactions

  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 11
  • Biochemical Activity: 5
  • Co-localization: 1
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 10

Genetic Interactions

  • Dosage Lethality: 1
  • Negative Genetic: 98
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 2
  • Positive Genetic: 8
  • Synthetic Growth Defect: 3
  • Synthetic Rescue: 9
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
RGT1 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Rgt1p has a role in regulation of glucose uptake. In the absence of glucose, Rgt1p binds to multiple consensus sites upstream of the promoters of its target genes to repress their transcription by recruiting the general repressors Cyc8p and Tup1p. The major targets of Rgt1p repression include the hexose transporter genes (HXT1-7) and the hexokinase gene HXK2. Rgt1p acts in concert with two paralogous corepressors, Mth1p and Std1p, which bind to it. In the presence of glucose, Mth1p and Std1p are phosphorylated by the protein kinase Yck1p, signaling their ubiquitination and subsequent degradation by the 26S proteasome. When Mth1p and Std1p are removed, Rgt1p is a target for phosphorylation by Protein Kinase A via the cAMP/PKA pathway. Phosphorylated Rgt1p is unable to bind DNA and therefore unable to repress transcription. Under high glucose conditions, Rgt1p is hyperphosphorylated, which converts it into a transcriptional activator; it is required under these conditions for full expression of HXT1.
Regulators
8
Targets
15
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
49
Additional
63
Reviews
25

Resources