FZO1 / YBR179C Overview


Standard Name
FZO1 1
Systematic Name
YBR179C
SGD ID
SGD:S000000383
Feature Type
ORF , Verified
Description
Mitofusin, protein involved in mitochondrial outer membrane fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system; activity regulated by ubiquitylation at conserved lysine residues and by deubiquitylases Ubp2p and Ubp12p 1 2 3 5 6 7 8 9
Name Description
FuZzy Onions homolog 4
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
FZO1 is located on chromosome II between EHT1 octanoyl-CoA:ethanol acyltransferase and DTR1 dityrosine transporter; coding sequence is 2568 nucleotides long with 18 SNPs, 3 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Mitofusin Fzo1p is 855 amino acids long, short-lived, and low in abundance; phosphorylated on 3 serines, ubiquitinylated on 5 lysines; shares domains with other proteins involved in organelle fission and mitochondrion organization
Length (a.a.)
855
Mol. Weight (Da)
97804.9
Isoelectric Point
7.0
Median Abundance (molecules/cell)
1772 +/- 495
Half-life (hr)
3.0

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all FZO1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
GTPase involved in mitochondrial fusion as a homodimer; localizes to both mitochondrial inner and outer membranes

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant has fragmented mitochondria, loses the mitochondrial genome, and cannot grow on nonfermentable carbon sources; overexpression also causes mitochondrial fragmentation; null mutant is sensitive to hyperosmotic stress and has lengthened replicative lifespan but shortened chronological lifespan; in large-scale studies, the null mutant has reduced competitive fitness, poor utilization of several nitrogen sources, and altered resistance to various chemicals
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
FZO1 is homologous to human MFN2, and has been used to study Charcot-Marie-Tooth disease type 2A (CMT2A)
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Fzo1p interacts physically with proteins involved in mitochondrion organization; FZO1 interacts genetically with genes involved in mitochondrion organization

224 total interactions for 157 unique genes

Physical Interactions

  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 29
  • Biochemical Activity: 1
  • Co-fractionation: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Lethality: 1
  • Dosage Rescue: 9
  • Negative Genetic: 75
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 16
  • Positive Genetic: 26
  • Synthetic Growth Defect: 10
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 8
  • Synthetic Rescue: 19
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
FZO1 promoter is bound by Gcn5p, Med6p, Rgr1p, Uga3p, Xbp1p, and Yap5p in response to heat; Fzo1p protein stability is downregulated by Mdm30p, Rsp5p, and Ubp12p; Fzo1p protein stability is upregulated by Ubp2p; Fzo1p protein stability is down regulated by Doa1p during replicative senescence
Regulators
15
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
60
Additional
65
Reviews
65

Resources