IRA1 / YBR140C Protein
Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval
for
various strains, sequence-based physico-chemical properties, protein modification sites, and external
identifiers for the protein.
Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.
AlphaFold Protein Structure
AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.
Experimental Data
Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.
Protein Half Life
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Protein Abundance
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| Abundance (molecules/cell) | Media | Treatment | Treatment time | Fold Change | Visualization | Strain background | Original Reference | Reference |
|---|---|---|---|---|---|---|---|---|
| 1512 | SD | untreated | confocal microscopy evidence | S288C | Breker M, et al. (2013) | Ho B, et al. (2018) | ||
| 1367 | SD | 2 mM 1,4-dithiothreitol | 2 hr | confocal microscopy evidence | S288C | Breker M, et al. (2013) | Ho B, et al. (2018) | |
| 1816 | SD minus nitrogen | cellular response to nitrogen starvation | 15 hr | confocal microscopy evidence | S288C | Breker M, et al. (2013) | Ho B, et al. (2018) | |
| 3236 | YEPD | untreated | flow cytometry evidence | S288C | Davidson GS, et al. (2011) | Ho B, et al. (2018) | ||
| 4900 | YEPD | cell quiescence | 7 d | 1.51 | flow cytometry evidence | S288C | Davidson GS, et al. (2011) | Ho B, et al. (2018) |
Domains and Classification - S288C
Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Protein Coordinates | Accession ID | Description | Source | No. of Genes with Domain |
|---|
Domain Locations
Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.
Scroll over a domain to view its exact coordinates and its Description.
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all IRA1 alleles in SGD search
Sequence
Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.
This locus is not translated into a protein.
1 MNQSDPQDKK NFPMEYSLTK HLFFDRLLLV LPIESNLKTY ADVEADSVFN SCRSIILNIA
61 ITKDLNPIIE NTLGLIDLIV QDEEITSDNI TDDIAHSILV LLRLLSDVFE YYWDQNNDFK
121 KIRNDNYKPG FSSHRPNFHT SRPKHTRINP ALATMLLCKI SKLKFNTRTL KVLQNMSHHL
181 SGSATISKSS ILPDSQEFLQ KRNYPAYTEK IDLTIDYIQR FISASNHVEF TKCVKTKVVA
241 PLLISHTSTE LGVVNHLDLF GCEYLTDKNL LAYLDILQHL SSYMKRTIFH SLLLYYASKA
301 FLFWIMARPK EYVKIYNNLI SSDYNSPSSS SDNGGSNNSD KTSISQLVSL LFDDVYSTFS
361 VSSLLTNVNN DHHYHLHHSS SSSKTTNTNS PNSISKTSIK QSSVNASGNV SPSQFSTGND
421 ASPTSPMASL SSPLNTNILG YPLSPITSTL GQANTSTSTT AATTKTDADT PSTMNTNNNN
481 NNNNSANLNN IPQRIFSLDD ISSFNSSRKS LNLDDSNSLF LWDTSQHSNA SMTNTNMHAG
541 VNNSQSQNDQ SSLNYMENIM ELYSNYTGSE LSSHTAILRF LVVLTLLDSE VYDEMNSNSY
601 RKISEPIMNI NPKDSNTSSW GSASKNPSIR HLTHGLKKLT LQQGRKRNVK FLTYLIRNLN
661 GGQFVSDVSL IDSIRSILFL MTMTSSISQI DSNIASVIFS KRFYNLLGQN LEVGTNWNSA
721 TANTFISHCV ERNPLTHRRL QLEFFASGLQ LDSDLFLRHL QLEKELNHID LPKISLYTEG
781 FRVFFHLVST KKLHEDIAEK TSSVLKRLFC IIADILLKAT PYFDDNVTKI IASILDGHIL
841 DQFDAARTLS NDDHVSFDAA TSVYTEPTEI IHNSSDASLV SSLSQSPLSI NSGSNITNTR
901 TWDIQSILPT LSNRSSASDL SLSNILTNPL EAQQNNNANL LAHRLSGVPT TKRYASPNDS
961 ERSRQSPYSS PPQLQQSDLP SPLSVLSSSA GFSSNHSITA TPTILKNIKS PKPNKTKKIA
1021 DDKQLKQPSY SRVILSDNDE ARKIMMNIFS IFKRMTNWFI RPDANTEFPK TFTDIIKPLF
1081 VSILDSNQRL QVTARAFIEI PLSYIATFED IDNDLDPRVL NDHYLLCTYA VTLFASSLFD
1141 LKLENAKREM LLDIIVKFQR VRSYLSNLAE KHNLVQAIIT TERLTLPLLV GAVGSGIFIS
1201 LYCSRGNTPR LIKISCCEFL RSLRFYQKYV GALDQYSIYN IDFIDAMAQD NFTASGSVAL
1261 QRRLRNNILT YIKGSDSILL DSMDVIYKKW FYFSCSKSVT QEELVDFRSL AGILASMSGI
1321 LSDMQELEKS KSAPDNEGDS LSFESRNPAY EVHKSLKLEL TKKMNFFISK QCQWLNNPNL
1381 LTRENSRDIL SIELHPLSFN LLFNNLGLKI DELMSIDLSK SHEDSSFVLL EQIIIIIRTI
1441 LKRDDDEKIM LLFSTDLLDA VDKLIEIVEK ISIKSSKYYK GIIQMSKMFR AFEHSEKNLG
1501 ISNHFHLKNK WLKLVIGWFK LSINKDYDFE NLSRPLREMD LQKRDEDFLY IDTSIESAKA
1561 LAYLTHNVPL EIPPSSSKED WNRSSTVSFG NHFTILLKGL EKSADLNQFP VSLRHKISIL
1621 NENVIIALTN LSNANVNVSL KFTLPMGYSP NKDIRIAFLR VFIDIVTNYP VNPEKHEMDK
1681 MLAIDDFLKY IIKNPILAFF GSLACSPADV DLYAGGFLNA FDTRNASHIL VTELLKQEIK
1741 RAARSDDILR RNSCATRALS LYTRSRGNKY LIKTLRPVLQ GIVDNKESFE IDKMKPGSEN
1801 SEKMLDLFEK YMTRLIDAIT SSIDDFPIEL VDICKTIYNA ASVNFPEYAY IAVGSFVFLR
1861 FIGPALVSPD SENIIIVTHA HDRKPFITLA KVIQSLANGR ENIFKKDILV SKEEFLKTCS
1921 DKIFNFLSEL CKIPTNNFTV NVREDPTPIS FDYSFLHKFF YLNEFTIRKE IINESKLPGE
1981 FSFLKNTVML NDKILGVLGQ PSMEIKNEIP PFVVENREKY PSLYEFMSRY AFKKVDMKEE
2041 EEDNAPFVHE AMTLDGIQII VVTFTNCEYN NFVMDSLVYK VLQIYARMWC SKHYVVIDCT
2101 TFYGGKANFQ KLTTLFFSLI PEQASSNCMG CYYFNVNKSF MDQWASSYTV ENPYLVTTIP
2161 RCFINSNTDQ SLIKSLGLSG RSLEVLKDVR VTLHDITLYD KEKKKFCPVS LKIGNKYFQV
2221 LHEIPQLYKV TVSNRTFSIK FNNVYKISNL ISVDVSNTTG VSSEFTLSLD NEEKLVFCSP
2281 KYLEIVKMFY YAQLKMEEDF GTDFSNDISF STSSSAVNAS YCNVKEVGEI ISHLSLVILV
2341 GLFNEDDLVK NISYNLLVAT QEAFNLDFGT RLHKSPETYV PDDTTTFLAL IFKAFSESST
2401 ELTPYIWKYM LDGLENDVIP QEHIPTVVCS LSYWVPNLYE HVYLANDEEG PEAISRIIYS
2461 LIRLTVKEPN FTTAYLQQIW FLLALDGRLT NVIVEEIVSH ALDRDSENRD WMKAVSILTS
2521 FPTTEIACQV IEKLINMIKS FLPSLAVEAS AHSWSELTIL SKISVSIFFE SPLLSQMYLP
2581 EILFAVSLLI DVGPSEIRVS LYELLMNVCH SLTNNESLPE RNRKNLDIVC ATFARQKLNF
2641 ISGFSQEKGR VLPNFAASSF SSKFGTLDLF TKNIMLLMEY GSISEGAQWE AKYKKYLMDA
2701 IFGHRSFFSA RAMMILGIMS KSHTSLFLCK ELLVETMKVF AEPVVDDEQM FIIIAHVFTY
2761 SKIVEGLDPS SELMKELFWL ATICVESPHP LLFEGGLLFM VNCLKRLYTV HLQLGFDGKS
2821 LAKKLMESRN FAATLLAKLE SYNGCIWNED NFPHIILGFI ANGLSIPVVK GAALDCLQAL
2881 FKNTYYERKS NPKSSDYLCY LFLLHLVLSP EQLSTLLLEV GFEDELVPLN NTLKVPLTLI
2941 NWLSSDSDKS NIVLYQGALL FSCVMSDEPC KFRFALLMRY LLKVNPICVF RFYTLTRKEF
3001 RRLSTLEQSS EAVAVSFELI GMLVTHSEFN YLEEFNDEMV ELLKKRGLSV VKPLDIFDQE
3061 HIEKLKGEGE HQVAIYERKR LATMILARMS CS*
* Blue amino acids indicate modification sites. More information below.
Post-translational Modifications - S288C
Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.
66 entries for 37 sitesIncrease the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.
| Site | Modification | Modifier | Reference |
|---|---|---|---|
| S4 | phosphorylated residue | Lanz MC, et al. (2021) PMID: 33491328 | |
| T62 | phosphorylated residue | Holt LJ, et al. (2009) PMID: 19779198 | |
| S330 | phosphorylated residue | Leutert M, et al. (2023) PMID: 37845410 | |
| S331 | phosphorylated residue | Leutert M, et al. (2023) PMID: 37845410 | |
| S331 | phosphorylated residue | Swaney DL, et al. (2013) PMID: 23749301 | |
| T388 | phosphorylated residue | Leutert M, et al. (2023) PMID: 37845410 | |
| T388 | phosphorylated residue | Lanz MC, et al. (2021) PMID: 33491328 | |
| S390 | phosphorylated residue | Lanz MC, et al. (2021) PMID: 33491328 | |
| S390 | phosphorylated residue | Leutert M, et al. (2023) PMID: 37845410 | |
| S390 | phosphorylated residue | Zhou X, et al. (2021) PMID: 33481703 |
Sequence-Based Physico-chemical Properties - S288C
Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.
Amino Acid Composition
Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.
| Amino Acid | Frequency | Percentage |
|---|---|---|
| A | 148 | 4.79 |
| C | 40 | 1.29 |
| D | 172 | 5.56 |
| E | 172 | 5.56 |
| F | 173 | 5.60 |
| G | 93 | 3.01 |
| H | 70 | 2.26 |
| I | 236 | 7.63 |
| K | 195 | 6.31 |
| L | 375 | 12.13 |
| M | 69 | 2.23 |
| N | 216 | 6.99 |
| P | 115 | 3.72 |
| Q | 83 | 2.68 |
| R | 115 | 3.72 |
| S | 349 | 11.29 |
| T | 176 | 5.69 |
| V | 164 | 5.30 |
| W | 23 | 0.74 |
| Y | 108 | 3.49 |
Physical Details
| Length (a.a): | 3092 |
| Molecular Weight (Da): | 350948.8 |
| Isoelectric Point (pl): | 6.54 |
| Formula: | C15796H24780N4094O4713S109 |
| Aliphatic Index: | 93.26 |
| Instability Index: | 43.77 |
Coding Region Translation Calculations
| Codon Bias: | 0.04 |
| Codon Adaptation Index: | 0.14 |
| Frequence of Optimal Codons: | 0.45 |
| Hydropathicity of Protein: | -0.12 |
| Aromaticity Score: | 0.1 |
Extinction Coefficients at 280nm
| ALL Cys residues appear as half cystines: | 289920.0 |
| NO Cys residues appear as half cystines: | 287420.0 |
Atomic Composition
Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.
| Atom | Frequency | Percentage |
|---|
Data not found or not available for S288C
External Identifiers
List of external identifiers for the protein from various database sources.
23 entries for 7 sourcesIncrease the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.
| External ID | Source |
|---|---|
| 2318 | DIP |
| AAA34709.1 | GenBank/EMBL/DDBJ |
| CAA53498.1 | GenBank/EMBL/DDBJ |
| CAA55537.1 | GenBank/EMBL/DDBJ |
| CAA85098.1 | GenBank/EMBL/DDBJ |
| X75891 | GenBank/EMBL/DDBJ |
| X78937 | GenBank/EMBL/DDBJ |
| 585325 | GenBank/EMBL/DDBJ |
| Z36009 | GenBank/EMBL/DDBJ |
| M24378 | GenBank/EMBL/DDBJ |
Resources
Homologs
AGD | AnalogYeast | BLASTP at NCBI | FungiDB | PhylomeDB | YGOB | YOGY
Protein Databases
AlphaFold Protein Structure | GPMDB | ModelArchive | Pfam domains | SUPERFAMILY | TopologYeast | UniProtKB
Localization
CYCLoPs | dHITS | LoQAtE | YeastGFP | YeastRC Public Images | YeastRGB | YPL+