MEC1 / YBR136W Protein
Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval
for
various strains, sequence-based physico-chemical properties, protein modification sites, and external
identifiers for the protein.
Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.
AlphaFold Protein Structure
AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.
Experimental Data
Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.
Protein Half Life
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Protein Abundance
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| Abundance (molecules/cell) | Media | Treatment | Treatment time | Fold Change | Visualization | Strain background | Original Reference | Reference |
|---|---|---|---|---|---|---|---|---|
| 894 | SD | untreated | quantitative mass spectrometry evidence | S288C | de Godoy LM, et al. (2008) | Ho B, et al. (2018) | ||
| 20 | YEPD | untreated | quantitative mass spectrometry evidence | S288C | Kulak NA, et al. (2014) | Ho B, et al. (2018) | ||
| 497 | YEPD | untreated | quantitative mass spectrometry evidence | Other | Lu P, et al. (2007) | Ho B, et al. (2018) | ||
| 224 | SC | untreated | quantitative mass spectrometry evidence | S288C | Peng M, et al. (2012) | Ho B, et al. (2018) | ||
| 55 | YEPD | untreated | quantitative mass spectrometry evidence | S288C | Webb KJ, et al. (2013) | Ho B, et al. (2018) |
Domains and Classification - S288C
Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Protein Coordinates | Accession ID | Description | Source | No. of Genes with Domain |
|---|
Domain Locations
Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.
Scroll over a domain to view its exact coordinates and its Description.
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MEC1 alleles in SGD search
Sequence
Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.
This locus is not translated into a protein.
1 MESHVKYLDE LILAIKDLNS GVDSKVQIKK VPTDPSSSQE YAKSLKILNT LIRNLKDQRR
61 NNIMKNDTIF SKTVSALALL LEYNPFLLVM KDSNGNFEIQ RLIDDFLNIS VLNYDNYHRI
121 WFMRRKLGSW CKACVEFYGK PAKFQLTAHF ENTMNLYEQA LTEVLLGKTE LLKFYDTLKG
181 LYILLYWFTS EYSTFGNSIA FLDSSLGFTK FDFNFQRLIR IVLYVFDSCE LAALEYAEIQ
241 LKYISLVVDY VCNRTISTAL DAPALVCCEQ LKFVLTTMHH FLDNKYGLLD NDPTMAKGIL
301 RLYSLCISND FSKCFVDHFP IDQWADFSQS EHFPFTQLTN KALSIVYFDL KRRSLPVEAL
361 KYDNKFNIWV YQSEPDSSLK NVTSPFDDRY KQLEKLRLLV LKKFNKTERG TLLKYRVNQL
421 SPGFFQRAGN DFKLILNEAS VSIQTCFKTN NITRLTSWTV ILGRLACLES EKFSGTLPNS
481 TKDMDNWYVC HLCDIEKTGN PFVRINPNRP EAAGKSEIFR ILHSNFLSHP NIDEFSESLL
541 SGILFSLHRI FSHFQPPKLT DGNGQINKSF KLVQKCFMNS NRYLRLLSTR IIPLFNISDS
601 HNSEDEHTAT LIKFLQSQKL PVVKENLVIA WTQLTLTTSN DVFDTLLLKL IDIFNSDDYS
661 LRIMMTLQIK NMAKILKKTP YQLLSPILPV LLRQLGKNLV ERKVGFQNLI ELLGYSSKTI
721 LDIFQRYIIP YAIIQYKSDV LSEIAKIMCD GDTSLINQMK VNLLKKNSRQ IFAVALVKHG
781 LFSLDILETL FLNRAPTFDK GYITAYLPDY KTLAEITKLY KNSVTKDASD SENANMILCS
841 LRFLITNFEK DKRHGSKYKN INNWTDDQEQ AFQKKLQDNI LGIFQVFSSD IHDVEGRTTY
901 YEKLRVINGI SFLIIYAPKK SIISALAQIS ICLQTGLGLK EVRYEAFRCW HLLVRHLNDE
961 ELSTVIDSLI AFILQKWSEF NGKLRNIVYS ILDTLIKEKS DLILKLKPYT TLALVGKPEL
1021 GILARDGQFA RMVNKIRSTT DLIPIFANNL KSSNKYVINQ NLDDIEVYLR RKQTERSIDF
1081 TPKKVGQTSD ITLVLGALLD TSHKFRNLDK DLCEKCAKCI SMIGVLDVTK HEFKRTTYSE
1141 NEVYDLNDSV QTIKFLIWVI NDILVPAFWQ SENPSKQLFV ALVIQESLKY CGLSSESWDM
1201 NHKELYPNEA KLWEKFNSVS KTTIYPLLSS LYLAQSWKEY VPLKYPSNNF KEGYKIWVKR
1261 FTLDLLKTGT TENHPLHVFS SLIREDDGSL SNFLLPYISL DIIIKAEKGT PYADILNGII
1321 IEFDSIFTCN LEGMNNLQVD SLRMCYESIF RVFEYCKKWA TEFKQNYSKL HGTFIIKDTK
1381 TTNMLLRIDE FLRTTPSDLL AQRSLETDSF ERSALYLEQC YRQNPHDKNQ NGQLLKNLQI
1441 TYEEIGDIDS LDGVLRTFAT GNLVSKIEEL QYSENWKLAQ DCFNVLGKFS DDPKTTTRML
1501 KSMYDHQLYS QIISNSSFHS SDGKISLSPD VKEWYSIGLE AANLEGNVQT LKNWVEQIES
1561 LRNIDDREVL LQYNIAKALI AISNEDPLRT QKYIHNSFRL IGTNFITSSK ETTLLKKQNL
1621 LMKLHSLYDL SFLSSAKDKF EYKSNTTILD YRMERIGADF VPNHYILSMR KSFDQLKMNE
1681 QADADLGKTF FTLAQLARNN ARLDIASESL MHCLERRLPQ AELEFAEILW KQGENDRALK
1741 IVQEIHEKYQ ENSSVNARDR AAVLLKFTEW LDLSNNSASE QIIKQYQDIF QIDSKWDKPY
1801 YSIGLYYSRL LERKKAEGYI TNGRFEYRAI SYFLLAFEKN TAKVRENLPK VITFWLDIAA
1861 ASISEAPGNR KEMLSKATED ICSHVEEALQ HCPTYIWYFV LTQLLSRLLH SHQSSAQIIM
1921 HILLSLAVEY PSHILWYITA LVNSNSSKRV LRGKHILEKY RQHSQNPHDL VSSALDLTKA
1981 LTRVCLQDVK SITSRSGKSL EKDFKFDMNV APSAMVVPVR KNLDIISPLE SNSMRGYQPF
2041 RPVVSIIRFG SSYKVFSSLK KPKQLNIIGS DGNIYGIMCK KEDVRQDNQY MQFATTMDFL
2101 LSKDIASRKR SLGINIYSVL SLREDCGILE MVPNVVTLRS ILSTKYESLK IKYSLKSLHD
2161 RWQHTAVDGK LEFYMEQVDK FPPILYQWFL ENFPDPINWF NARNTYARSY AVMAMVGHIL
2221 GLGDRHCENI LLDIQTGKVL HVDFDCLFEK GKRLPVPEIV PFRLTPNLLD ALGIIGTEGT
2281 FKKSSEVTLA LMRKNEVALM NVIETIMYDR NMDHSIQKAL KVLRNKIRGI DPQDGLVLSV
2341 AGQTETLIQE ATSEDNLSKM YIGWLPFW*
* Blue amino acids indicate modification sites. More information below.
Post-translational Modifications - S288C
Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.
27 entries for 14 sitesIncrease the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.
| Site | Modification | Modifier | Reference |
|---|---|---|---|
| S36 | phosphorylated residue | Zhou X, et al. (2021) PMID: 33481703 | |
| S36 | phosphorylated residue | Leutert M, et al. (2023) PMID: 37845410 | |
| S37 | phosphorylated residue | Albuquerque CP, et al. (2008) PMID: 18407956 | |
| S37 | phosphorylated residue | Lanz MC, et al. (2021) PMID: 33491328 | |
| S37 | phosphorylated residue | Swaney DL, et al. (2013) PMID: 23749301 | |
| S38 | phosphorylated residue | TEL1 | Chen SH, et al. (2010) PMID: 20190278 |
| S38 | phosphorylated residue | Albuquerque CP, et al. (2008) PMID: 18407956 | |
| S38 | phosphorylated residue | Leutert M, et al. (2023) PMID: 37845410 | |
| S38 | phosphorylated residue | MEC1 | Chen SH, et al. (2010) PMID: 20190278 |
| S38 | phosphorylated residue | Chen SH, et al. (2010) PMID: 20190278 |
Sequence-Based Physico-chemical Properties - S288C
Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.
Amino Acid Composition
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| Amino Acid | Frequency | Percentage |
|---|---|---|
| A | 109 | 4.60 |
| C | 34 | 1.44 |
| D | 140 | 5.91 |
| E | 132 | 5.57 |
| F | 120 | 5.07 |
| G | 85 | 3.59 |
| H | 48 | 2.03 |
| I | 183 | 7.73 |
| K | 186 | 7.85 |
| L | 304 | 12.84 |
| M | 45 | 1.90 |
| N | 142 | 6.00 |
| P | 71 | 3.00 |
| Q | 96 | 4.05 |
| R | 105 | 4.43 |
| S | 198 | 8.36 |
| T | 127 | 5.36 |
| V | 114 | 4.81 |
| W | 32 | 1.35 |
| Y | 97 | 4.10 |
Physical Details
| Length (a.a): | 2368 |
| Molecular Weight (Da): | 273363.7 |
| Isoelectric Point (pl): | 8.45 |
| Formula: | C12380H19500N3235O3573S79 |
| Aliphatic Index: | 93.44 |
| Instability Index: | 38.77 |
Coding Region Translation Calculations
| Codon Bias: | 0.03 |
| Codon Adaptation Index: | 0.14 |
| Frequence of Optimal Codons: | 0.45 |
| Hydropathicity of Protein: | -0.22 |
| Aromaticity Score: | 0.11 |
Extinction Coefficients at 280nm
| ALL Cys residues appear as half cystines: | 322655.0 |
| NO Cys residues appear as half cystines: | 320530.0 |
Atomic Composition
Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.
| Atom | Frequency | Percentage |
|---|
Data not found or not available for S288C
External Identifiers
List of external identifiers for the protein from various database sources.
25 entries for 9 sourcesIncrease the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.
| External ID | Source |
|---|---|
| orf19.1283 | CGD |
| 799 | DIP |
| 2.7.11.1 | ExPASy |
| U31109 | GenBank/EMBL/DDBJ |
| D11088 | GenBank/EMBL/DDBJ |
| X75891 | GenBank/EMBL/DDBJ |
| 398365009 | GenBank/EMBL/DDBJ |
| Z36005 | GenBank/EMBL/DDBJ |
| AAA74482.1 | GenBank/EMBL/DDBJ |
| CAA53494.1 | GenBank/EMBL/DDBJ |
Resources
Homologs
AGD | AnalogYeast | BLASTP at NCBI | CGD | FungiDB | PhylomeDB | PomBase | YGOB | YOGY
Protein Databases
AlphaFold Protein Structure | GPMDB | ModelArchive | Pfam domains | SUPERFAMILY | TopologYeast | UniProtKB
Localization
CYCLoPs | dHITS | LoQAtE | YeastGFP | YeastRGB | YPL+