New & Noteworthy

Mixing Mitochondria Makes Magic

May 31, 2018


In the Harry Potter universe, there are two materials that make up a wand: the wood, which comes from trees like cedar and holly, and the core, which is a magical substance such as the feather of a phoenix or a unicorn hair.

OllivandersWands

A variety of wizarding wands. From Flickr.

Every wand has unique properties that depend mainly on the combination of its wood and core. Different wood-core pairings give different characteristics that can either antagonize or synergize with the wizard using it. When a wand meets up with its ideal owner, it will begin to learn from and teach its human partner. Such auspicious pairings can continuously improve the wizard’s spell-casting, helping the wizard perform better and better under ever more varied circumstances. However, a poor pairing between a wand and a wizard can be devastating, enfeebling the wizard’s magic or even causing it to backfire.

And just like wizards and wands, it turns out that mitochondrial DNA and nuclear DNA in a cell need to be properly paired to perform the “magic” of running a cell in the most efficient way.

Mitochondria are dynamic structures inside eukaryotic cells that provide much of the energy to keep a cell humming along.

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A mitochondrion. These sub-cellular organelles produce much of the energy for a cell in the form of ATP. From Wikimedia Commons.

Mitochondria contain their own DNA, encoding genes necessary for the organelle to do its work. Although mitochondrial DNA is physically separate from nuclear DNA, it turns out that the two need to work together if the cell is to make functioning mitochondria.

Like the wand-wizard pairing in Harry Potter’s world, the combination of a specific mitochondrial genome (the wand) with a particular nuclear genome (the wizard) is important for making a healthy mitochondrion. Some mitochondrial-nuclear combinations work well and others not so much, but not a lot is known about where different mitochondrial DNAs come from and how they end up paired with their favored nuclear genomes.

Knowing more about this may help us understand how mitochondrial genomes evolve during interspecific hybridizations, such as in lager beer yeast and certain other fermentation yeasts.

A new study in GENETICS from Wolters et al. shows that when S. cerevisiae yeast cells go through the mating process, there is often mixing of mitochondrial genomes to give new combinations of mitochondrial genes — almost as if lots of new wood-core combinations of wands were being created.

How do these new mitochondrial combinations arise? When two haploid yeast cells mate, they merge to form a single diploid cell that contains mitochondria from both of its parents. Sometimes, these mitochondria exchange pieces of DNA, mixing-and-matching genes in a process known as mitochondrial recombination.

The authors found that a surprising proportion of mated yeast cells (~40%) had recombinant mitochondrial DNA. And in many cases, the recombined mitochondrial genomes work even better with the nuclear genome to make a super healthy cell. Often these optimal pairings allowed the cells to develop new powers, tolerating higher temperatures and more oxygen-stressed conditions than the original parent cells — in other words, the cell has found its optimal “wand”!

But other pairing combinations were inauspicious, giving sickly or dead mitochondria that can harm the cell, especially when it is growing under stressful conditions. For instance, when the authors swapped the mitochondrial-nuclear pairing for two different but very fit cell types, these new pairings gave unhealthy cells, meaning that the original fit cells had already found their perfect “wand”.

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Harry Potter first meeting his perfectly-paired wand. From harrypotter.wikia.com.

So just like when Harry Potter was in Ollivander’s wand shop and finally found his holly-phoenix feather wand and felt unified with its amazing magic, yeast cells can acquire new super powers when their nuclear and mitochondrial genomes are perfectly paired!

by Barbara Dunn, Ph.D. and Kevin MacPherson, M.S.

…and we wish a fond farewell to Barry Starr, Ph.D. who has left the Stanford Department of Genetics for new horizons. We miss you Barry and wish you well!

Categories: Research Spotlight

Tags: recombination, mitochondria, environmental stress

Affecting the Shelf Life of Chromosomes

December 19, 2013

Just like the chicken or milk you buy at a store, chromosomes have a shelf life too.  Of course, chromosomes don’t spoil because of growing bacteria.  Instead, they go bad because they lose a little of the telomeres at their ends each time they are copied.  Once these telomeres get too short, the chromosome stops working and the cell dies.

You can make this chicken last longer by freezing it. You can do the same for a chromosome in yeast with a shot of alcohol. Image from Food & Spirits Magazine via Wikimedia Commons

Turns out food and chromosomes have another thing in common—the rates of spoilage of both can be affected by their environment.   For example, we all know that chicken will last longer if you store it in a refrigerator and that it will go bad sooner if you leave it out on the counter on a hot day.  In a new study out in PLoS Genetics, Romano and coworkers show a variety of ways that the loss of telomeres can be slowed down or sped up in the yeast S. cerevisiae.  And importantly, they also show that some forms of environmental stress have no effect.

The authors looked at the effect of thirteen different environments on telomere length over 100-400 generations.  They found that caffeine, high temperature and low levels of hydroxyurea lead to shortened telomeres, while alcohol and acetic acid lead to longer telomeres.  It seems that for a long life, yeast should lay off the espresso and and try to avoid fevers, while enjoying those martinis and sauerbraten.

Romano and coworkers also found a number of conditions that had no effect on telomere length, with the most significant being oxidative stress.  In contrast, previous studies in humans had suggested that the oxidative stress associated with emotional stress contributed to increased telomere loss; given these results, this may need to be looked at again.  In any event, yeast can deal with the stresses of modern life with little or no impact on their telomere length.

The authors next set out to identify the genes that are impacted by these stressors.  They focused on four different conditions—two that led to decreased telomere length, high temperature and caffeine, one that led to longer telomeres, ethanol, and one that had no effect, hydrogen peroxide.  As a first step they identified key genes by comparing genome-wide transcript levels under each condition.  They then went on to look at the effect of each stressor on strains deleted for each of the genes they identified.

Not surprisingly, the most important genes were those involved with the enzyme telomerase.  This enzyme is responsible for adding to the telomeres at the ends of chromosomes.  Without something like this, eukaryotes, with their linear chromosomes, would have disappeared long ago.

A key gene they identified was RIF1, encoding a negative regulator of telomerase.  Deleting this gene led to decreased effects of ethanol and caffeine, suggesting that this gene is key to each stressor’s effects.  The same was not true of high temperature—the strain deleted for RIF1 responded normally to high temperature.  So high temperature works through a different mechanism.

Digging deeper into this pathway, Romano and coworkers found that Rap1p was the central player in ethanol’s ability to lengthen telomeres.  This makes sense, as the ability of Rif1p to negatively regulate telomerase depends upon its interaction with Rap1p. 

The increase in telomere length by ethanol was not just dependent on genes associated with telomerase either.  The authors identified a number of other genes involved, including DOA4, SNF7, and DID4

Caffeine, like ethanol, affected telomere length through Rif1p-Rap1p but with an opposite effect.  As caffeine is known to be an inhibitor of phosphatydylinositol-3 kinase related kinases, the authors looked at whether known kinases in the telomerase pathway were involved in caffeine-dependent telomere shortening.  They found that when they deleted both TEL1 and MEC1, caffeine no longer affected telomere length. 

The authors were not so lucky in their attempts to tease out the mechanism of the ability of high temperature to shorten telomeres.  They were not able to identify any single deletions that eliminated this effect of high temperature.

Whatever the mechanisms, the results presented in this study are important for a couple of different reasons.  First off, they obviously teach us more about how telomere length is maintained.  But this is more than a dry, academic finding.

Given that many of the 400 or so genes involved in maintaining telomere length are evolutionarily conserved, these results may also translate to humans too.  This matters because telomere length is involved in a number of diseases and aging.

Studies like this may help us identify novel genes to target in diseases like cancer.  And they may help us better understand how lifestyle choices can affect your telomeres and so your health.  So if you have a cup of coffee, be sure to spike it with alcohol! 

by D. Barry Starr, Ph.D., Director of Outreach Activities, Stanford Genetics

Categories: Research Spotlight

Tags: Saccharomyces cerevisiae, environmental stress, telomere

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