Nepetalactone, a natural insect repellent comparable to N,N-diethyl-meta-toluamide (DEET), is challenging to produce through plant extraction or chemical synthesis. This study achieved the de novo synthesis of nepetalactone in Saccharomyces cerevisiae without expensive precursors or inducers. Initially, the metabolic pathway for nepetalactone synthesis was successfully established in Saccharomyces cerevisiae. A metabolic pathway was established using strategies such as iridoid synthase (ISY) source screening, enzyme fusion, and cofactor regeneration to optimize nepetalactone production. Bioprocess optimization through chromosomal integration and two-phase fermentation prevented its conversion to dihydronepetalactone, resulting in a high-yield strain, NTE21, with a titer of 2.5 g/L. A record titer of 4.5 g/L was achieved in 5.0 L fed-batch fermentation via continuous batch feeding. This study documents the potential of microbial platforms for the sustainable, cost-effective, and scalable production of nepetalactone, paving the way for its commercial application as a natural insect repellent.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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