The vast majority of eukaryotic promoter regions are divergently transcribed. While divergent transcription serves a key role in shaping the transcriptome, a structural basis for this process remains unexplored. Whether two transcription pre-initiation complexes (PICs) can co-assemble at divergent promotes, and if so, what structural relationships may emerge therefrom, remains unknown. To investigate the structural bases of this phenomenon, we pursued the assembly of the Saccharomyces cerevisiae Mediator-PIC on a divergent promoter. Taking together observations from Cryo-EM, crosslinking-mass spectrometry, and in vitro transcription assays, we were able to capture two distinct PICs co-assembled at divergent promoters, consistent with recent emerging model derived from in vivo observations. Overall, the structure of the Mediator-PIC in this novel form is conducive to transcriptional activation for divergent transcription.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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