The effects of laccase pretreatment and surfactant addition in the simultaneous saccharification and fermentation (SSF) of corn stover by engineered Saccharomyces cerevisiae were studied. Surfactants Tween-80, tea saponin and rhamnolipid improved ethanol production in SSF, among which the biosurfactant rhamnolipid reached the highest ethanol yield. At the 6 d in SSF, the ethanol content of addition rhamnolipid of laccase pretreatment corn stover (Lac-CS) and Lac-CS reached 0.73 g/L and 0.56 g/L, which was 2.32 folds and 1.54 folds higher than the control of 0.22 g/L, respectively. These findings suggested that the combination of laccase pretreatment and rhamnolipid addition further improve ethanol production. GC-MS, composition of corn stover, protein concentration of supernatant and glucose content studies were executed to explore the mechanism of combination strategy of laccase pretreatment and rhamnolipid addition enhance ethanol production. This study provides guidance for the application of laccase and surfactant in bioethanol production.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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