Protein-RNA interaction participates in many biological processes. So, studying protein-RNA interaction can help us to understand the function of protein and RNA. Although the protein-RNA 3D3D model, like PRIME, was useful in building 3D structural complexes, it can't be used genome-wide, due to lacking RNA 3D structures. To take full advantage of RNA secondary structures revealed from high-throughput sequencing, we present PRIME-3D2D to predict binding sites of protein-RNA interaction. PRIME-3D2D is almost as good as PRIME at modeling protein-RNA complexes. PRIME-3D2D can be used to predict binding sites on PDB data (MCC = 0.75/0.70 for binding sites in protein/RNA) and transcription-wide (MCC = 0.285 for binding sites in RNA). Testing on PDB and yeast transcription-wide data show that PRIME-3D2D performs better than other binding sites predictor. So, PRIME-3D2D can be used to predict the binding sites both on PDB and genome-wide, and it's freely available.

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Journal Article | Research Support, Non-U.S. Gov't

Authors

Xie J, Zheng J, Hong X, Tong X, Liu S

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