The switch from gluconeogenesis to glycolysis in yeast has been shown to require ubiquitin-proteasome dependent elimination of the key enzyme fructose-1,6-bisphosphatase (FBPase). Prior to proteasomal degradation, polyubiquitination of the enzyme occurs via the ubiquitin-conjugating enzymes Ubc1, Ubc4, Ubc5 and Ubc8 in conjunction with a novel multi-subunit ubiquitin ligase, the Gid complex. As an additional machinery required for the catabolite degradation process, we identified the trimeric Cdc48(Ufd1-Npl4) complex and the ubiquitin receptors Dsk2 and Rad23. We show that this machinery acts between polyubiquitination of FBPase and its degradation by the proteasome.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| NPL4 | involved in | proteasome-mediated ubiquitin-dependent protein catabolic process | IMP | SGD | 2013-08-07 | |
| CDC48 | involved in | proteasome-mediated ubiquitin-dependent protein catabolic process | IMP | SGD | 2013-08-07 | |
| UFD1 | involved in | proteasome-mediated ubiquitin-dependent protein catabolic process | IMP | SGD | 2013-08-07 |
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| RAD23 | protein/peptide accumulation: increased Reporter: Fbp1p | classical genetics | null Allele: rad23-Δ | Other | Media: ethanol grown cells shifted to glucose Details: delayed degradation of FBPase after shift from gluconeogenic to glycolytic growth | |
| UBX4 | protein/peptide accumulation: increased Reporter: Fbp1p | classical genetics | null Allele: ubx4-Δ | Other | Media: ethanol grown cells shifted to glucose Details: delayed degradation of FBPase after shift from gluconeogenic to glycolytic growth | |
| DSK2 | protein/peptide accumulation: increased Reporter: Fbp1p | classical genetics | null Allele: dsk2-Δ | Other | Media: ethanol grown cells shifted to glucose Details: delayed degradation of FBPase after shift from gluconeogenic to glycolytic growth | |
| CDC48 | protein/peptide accumulation: increased Reporter: Fbp1p | classical genetics | conditional Allele: cdc48-ts T413R | W303 | Media: ethanol grown cells shifted to glucose Temperature: elevated temperature, 37 °C Details: decreased degradation of FBPase (and Pyc1/2p) after shift from gluconeogenic to glycolytic conditions | |
| UFD1 | protein/peptide accumulation: increased Reporter: Fbp1p | classical genetics | conditional Allele: ufd1-1 V94D | Other | Media: ethanol grown cells shifted to glucose Temperature: elevated temperature, 37 °C Details: decreased degradation of FBPase (and Pyc1/2p) after shift from gluconeogenic to glycolytic conditions | |
| NPL4 | protein/peptide accumulation: increased Reporter: Fbp1p | classical genetics | conditional Allele: npl4-2 W569* (residue position corrected); results in a truncated protein lacking the C-terminal 12 amino acids | Other | Media: ethanol grown cells shifted to glucose Temperature: elevated temperature, 37 °C Details: decreased degradation of FBPase (and Pyc1/2p) after shift from gluconeogenic to glycolytic conditions |