A cDNA sub-library from the fungus Trichoderma reesei grown on xylan was constructed in S.cerevisiae recombinant strain H475 harboring a xylose reductase (XR) gene from Pichia stipitis. The xylitol dehydrogenase (XDH) gene was screened from sub-library on a SC selective medium in which xylose was used as a sole carbon source. The length of XDH gene, xdh1, was about 1.3 kb. Southern, Northern and Western blots were carried out, indicating that xdh1 has high affinity with T.reesei and the molecular weight of the xylitol dehydrogenase from T.reesei was about 40 kDa. The strain HX1 harboring both the XR gene from P.stipitis and the XDH gene from T.reesei was able to grow on the xylose medium and converted more than 90% of the xylose into xylitol, ethanol, and other by-products.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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