Reference: Ruiz A, et al. (2006) Role of protein phosphatases 2C on tolerance to lithium toxicity in the yeast Saccharomyces cerevisiae. Mol Microbiol 62(1):263-77

Reference Help

Abstract


Protein phosphatases 2C are a family of conserved enzymes involved in many aspects of the cell biology. We reported that, in the yeast Saccharomyces cerevisiae, overexpression of the Ptc3p isoform resulted in increased lithium tolerance in the hypersensitive hal3 background. We have found that the tolerance induced by PTC3 overexpression is also observed in wild-type cells and that this is most probably the result of increased expression of the ENA1 Na(+)-ATPase mediated by the Hog1 MAP kinase pathway. This effect does not require a catalytically active protein. Surprisingly, deletion of PTC3 (similarly to that of PTC2, PTC4 or PTC5) does not confer a lithium-sensitive phenotype, but mutation of PTC1 does. Lack of PTC1 in an ena1-4 background did not result in additive lithium sensitivity and the ptc1 mutant showed a decreased expression of the ENA1 gene in cells stressed with LiCl. In agreement, under these conditions, the ptc1 mutant was less effective in extruding Li(+) and accumulated higher concentrations of this cation. Deletion of PTC1 in a hal3 background did not exacerbate the halosensitive phenotype of the hal3 strain. In addition, induction from the ENA1 promoter under LiCl stress decreased similarly (50%) in hal3, ptc1 and ptc1 hal3 mutants. Finally, mutation of PTC1 virtually abolishes the increased tolerance to toxic cations provided by overexpression of Hal3p. These results indicate that Ptc1p modulates the function of Ena1p by regulating the Hal3/Ppz1,2 pathway. In conclusion, overexpression of PTC3 and lack of PTC1 affect lithium tolerance in yeast, although through different mechanisms.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Ruiz A, González A, García-Salcedo R, Ramos J, Ariño J
Primary Lit For
CNB1 | PTC1 | PTC3 | SIS2
Additional Lit For
TRK2 | PTC2 | ENA1 | HOG1 | PPZ1 | SKO1 | TRK1

Phenotype Annotations 10 entries for 4 genes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
CNB1ionic stress resistance: decreased
classical geneticsnull
Allele: cnb1-Δ
S288C lithium(1+)Treatment: lithium chloride, 35-50 mM
PTC1ionic stress resistance: decreased
classical geneticsnull
Allele: ptc1-Δ
S288C lithium(1+)Treatment: lithium chloride, 50-150 mM
PTC1ionic stress resistance: decreased
classical geneticsnull
Allele: ptc1-Δ
S288C0.25 M tetramethylammonium
SIS2ionic stress resistance: decreased
classical geneticsnull
Allele: sis2-Δ
S288C lithium(1+)Treatment: lithium chloride, 50-150 mM
PTC3ionic stress resistance: increased
classical geneticsoverexpressionOther lithium(1+)Treatment: lithium chloride, 150-200 mM
SIS2ionic stress resistance: increased
classical geneticsoverexpressionS288C lithium(1+)Treatment: lithium chloride, 75-500 mM
SIS2ionic stress resistance: increased
classical geneticsoverexpressionS288C0.5 M tetramethylammonium
PTC1resistance to chemicals: decreased
classical geneticsnull
Allele: ptc1-Δ
S288C0.25 mM spermine
SIS2resistance to chemicals: increased
classical geneticsoverexpressionS288C0.5-0.75 mM spermine
PTC1small molecule transport: decreased
classical geneticsnull
Allele: ptc1-Δ
S288C lithium(1+)Treatment: lithium chloride, 100 mM
Details: cells loaded with 100 mM LiCl
Showing 1 to 10 of 10 entries

Interaction Annotations


Genetic Interactions

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 0 entries for 0 genes

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

InteractorInteractorAssayAnnotationActionModification
No physical interaction data for Ruiz A, et al. (2006)
Showing 0 to 0 of 0 entries