HOG1 / YLR113W Overview

Standard Name
HOG1 1
Systematic Name
SSK3 29
Feature Type
ORF , Verified
Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNAPII during osmotic shock; mediates recruitment/activation of RNAPII at Hot1p-dependent promoters; binds calmodulin; stimulates antisense transcription to activate CDC28; defines novel S-phase checkpoint with Mrc1p that prevent replication/transcription conflicts; nuclear form represses pseudohyphal growth; autophosphorylates; protein abundance increases under DNA replication stress 1 2 3 4 5 6 7 8 9 10 11
Name Description
High Osmolarity Glycerol response 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

HOG1 is on the right arm of chromosome XII between uncharacterized gene YLR112W and replication origin ARS1213; coding sequence is 1308 nucleotides long with 9 SNPs, 7 of which are silent, one which leads to a Ser/Leu polymorphism at residue 302 and another which leads to an Ile/Val polymorphism at residue 346
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Hog1p is 435 amino acids long with 4 P38 MAP kinase signature domains; shares domains with proteins involved in invasive growth; autophosphorylates; undergoes various post-translational modifications, including phosphorylation, acetylation, succinylation, and ubiquitinylation at 7 sites; protein abundance increases under DNA replication stress
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
7457 +/- 2086
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all HOG1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Calmodulin- and chromatin-binding mitogen-activated protein kinase involved in osmoregulation, downregulation of mitotic exit, and regulation of various processes including transcription, DNA replication, and macroautophagy; localizes to both nucleus and cytoplasm

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant is sensitive to hyperosmotic stress and fails to accumulate glycerol under hyperosmotic conditions; null mutation confers increased sensitivity to cold, acid and multiple chemicals including tunicamycin, DTT, SDS, Congo Red

Classical Genetics

reduction of function
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Interacts physcially with proteins involved in transcription; interacts genetically with genes involved in transcription

1137 total interactions for 696 unique genes

Physical Interactions

  • Affinity Capture-MS: 183
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 71
  • Biochemical Activity: 51
  • Co-localization: 45
  • Co-purification: 1
  • PCA: 11
  • Proximity Label-MS: 13
  • Reconstituted Complex: 5
  • Two-hybrid: 19

Genetic Interactions

  • Dosage Growth Defect: 81
  • Dosage Lethality: 1
  • Dosage Rescue: 25
  • Negative Genetic: 362
  • Phenotypic Enhancement: 24
  • Phenotypic Suppression: 89
  • Positive Genetic: 25
  • Synthetic Growth Defect: 57
  • Synthetic Haploinsufficiency: 2
  • Synthetic Lethality: 8
  • Synthetic Rescue: 60
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Protein activity is upregulated during heat response by Slt2p, Ssk1p, Ssk2p, and Ssk22p; transcription is downregulated during hypoxic response by Ixr1p; promoter is bound by Med2p and Spt3p during heat response, and by Sfp1p during stress response
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2006-10-18

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.