The accurate segregation of chromosomes requires the kinetochore, a complex protein machine that assembles onto centromeric DNA to mediate attachment of replicated sister chromatids to the mitotic spindle apparatus. This study reveals an important role for the yeast RSC ATP-dependent chromatin-remodeling complex at the kinetochore in chromosome transmission. Mutations in genes encoding two core subunits of RSC, the ATPase Sth1p and the Snf5p homolog Sfh1p, interact genetically with mutations in genes encoding kinetochore proteins and with a mutation in centromeric DNA. RSC also interacts genetically and physically with the histone and histone variant components of centromeric chromatin. Importantly, RSC is localized to centromeric and centromere-proximal chromosomal regions, and its association with these loci is dependent on Sth1p. Both sth1 and sfh1 mutants exhibit altered centromeric and centromere-proximal chromatin structure and increased missegregation of authentic chromosomes. Finally, RSC is not required for centromeric deposition of the histone H3 variant Cse4p, suggesting that RSC plays a role in reconfiguring centromeric and flanking nucleosomes following Cse4p recruitment for proper chromosome transmission.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Interactor | Interactor | Assay | Annotation | Action | Modification | |
|---|---|---|---|---|---|---|
| HHF1 | STH1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| HHF1 | STH1 | Far Western | manually curated | Bait-Hit | No Modification | |
| HHF1 | STH1 | Reconstituted Complex | manually curated | Bait-Hit | No Modification | |
| HHT1 | STH1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| HHT1 | STH1 | Far Western | manually curated | Bait-Hit | No Modification | |
| HHT1 | STH1 | Reconstituted Complex | manually curated | Bait-Hit | No Modification | |
| STH1 | HTB1 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| STH1 | HTB1 | Far Western | manually curated | Hit-Bait | No Modification | |
| STH1 | HTB1 | Reconstituted Complex | manually curated | Hit-Bait | No Modification | |
| STH1 | RSC8 | Two-hybrid | manually curated | Bait-Hit | No Modification |