Reference: Neely KE, et al. (1999)
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Abstract
The yeast SWI/SNF complex is required for the transcription of several yeast genes and has been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we show that the complex stimulated in vitro transcription from nucleosome templates in an activation domain-dependent manner. Transcription stimulation by SWI/SNF required an activation domain with which it directly interacts. The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts. The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't |
Research Support, U.S. Gov't, P.H.S.
- Authors
-
Neely KE,
Hassan AH,
Wallberg AE,
Steger DJ,
Cairns BR,
Wright AP,
Workman JL
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Gene Ontology Annotations
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| Evidence ID |
Analyze ID |
Gene/Complex |
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
|---|
| GCN4 | SNF2 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| GCN4 | ARP7 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| GCN4 | TAF14 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| HAP4 | SNF2 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| HAP4 | ARP7 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| HAP4 | TAF14 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| SWI5 | ARP7 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
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