Reference: Neely KE, et al. (1999) Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays. Mol Cell 4(4):649-55

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Abstract


The yeast SWI/SNF complex is required for the transcription of several yeast genes and has been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we show that the complex stimulated in vitro transcription from nucleosome templates in an activation domain-dependent manner. Transcription stimulation by SWI/SNF required an activation domain with which it directly interacts. The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts. The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Neely KE, Hassan AH, Wallberg AE, Steger DJ, Cairns BR, Wright AP, Workman JL
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Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 7 entries for 6 genes

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InteractorInteractorAssayAnnotationActionModification
GCN4SNF2Affinity Capture-Westernmanually curatedBait-HitNo Modification
GCN4ARP7Affinity Capture-Westernmanually curatedBait-HitNo Modification
GCN4TAF14Affinity Capture-Westernmanually curatedBait-HitNo Modification
HAP4SNF2Affinity Capture-Westernmanually curatedBait-HitNo Modification
HAP4ARP7Affinity Capture-Westernmanually curatedBait-HitNo Modification
HAP4TAF14Affinity Capture-Westernmanually curatedBait-HitNo Modification
SWI5ARP7Affinity Capture-Westernmanually curatedBait-HitNo Modification
Showing 1 to 7 of 7 entries