Reference: Milkereit P, et al. (2001)
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Abstract
How pre-ribosomes temporally and spatially mature during intranuclear biogenesis is not known. Here, we report three nucleolar proteins, Noc1p to Noc3p, that are required for ribosome maturation and transport. They can be isolated in two distinct complexes: Noc1p/Noc2p associates with 90S and 66S pre-ribosomes and is enriched in the nucleolus, and Noc2p/Noc3p associates with 66S pre-ribosomes and is mainly nucleoplasmic. Mutation of each Noc protein impairs intranuclear transport of 60S subunits at different stages and inhibits pre-rRNA processing. Overexpression of a conserved domain common to Noc1p and Noc3p is dominant-negative for cell growth, with a defect in nuclear 60S subunit transport, but no inhibition of pre-rRNA processing. We propose that the dynamic interaction of Noc proteins is crucial for intranuclear movement of ribosomal precursor particles, and, thereby represent a prerequisite for proper maturation.
- Reference Type
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Journal Article
- Authors
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Milkereit P,
Gadal O,
Podtelejnikov A,
Trumtel S,
Gas N,
Petfalski E,
Tollervey D,
Mann M,
Hurt E,
Tschochner H
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- MAK21 | NOC3 | NOC2 | NOC1-NOC2 pre-ribosome maturation complex | NOC2-NOC3 pre-ribosome maturation complex
- NOC4
Gene Ontology Annotations
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| Evidence ID |
Analyze ID |
Gene/Complex |
Systematic Name/Complex Accession |
Qualifier |
Gene Ontology Term ID |
Gene Ontology Term |
Aspect |
Annotation Extension |
Evidence |
Method |
Source |
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Reference |
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| Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Phenotype |
Experiment Type |
Experiment Type Category |
Mutant Information |
Strain Background |
Chemical |
Details |
Reference |
Disease Annotations
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| Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Disease Ontology Term |
Disease Ontology Term ID |
Qualifier |
Evidence |
Method |
Source |
Assigned On |
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Reference |
Regulation Annotations
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| Evidence ID |
Analyze ID |
Regulator |
Regulator Systematic Name |
Target |
Target Systematic Name |
Direction |
Regulation of |
Happens During |
Regulator Type |
Direction |
Regulation Of |
Happens During |
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Evidence |
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Reference |
Post-translational Modifications
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Genetic Interactions
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| Evidence ID |
Analyze ID |
|
Interactor |
Interactor Systematic Name |
Interactor |
Interactor Systematic Name |
Allele |
Assay |
Annotation |
Action |
Phenotype |
SGA score |
P-value |
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Reference |
Note |
Physical Interactions
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
|---|
| MAK21 | NOC2 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| MAK21 | NOC2 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| NOC3 | NOC2 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| NOC3 | NOC2 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
Functional Complementation Annotations
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| Complement ID |
Locus ID |
Gene |
Species |
Gene ID |
Strain background |
Direction |
Details |
Source |
Reference |